纤维素降解菌的分离、鉴定及其产酶特性研究

Research on Isolation, Identification and Enzyme Production Characterization of Cellulose Degrading Bacteria

从纤维素富集的环境中分离到3株能以羧甲基纤维素钠(CMC-Na)为唯一碳源的纤维素降解菌,采用纤维素-刚果红染色法进一步筛选,获得透明圈较大的菌株1株,命名为为B1。经菌落、菌体形态观察、生理生化实验及分子生物学鉴定,初步确定为蜡样芽胞杆菌(Bacillus cereus),该菌株表现出了较高的滤纸崩解能力和较高的纤维素酶活力。通过培养条件的优化,发现该菌株在起始p H值为5.0的培养基中,37℃培养36 h,其纤维素酶活力可达64.22 U/m L。

Three cellulose degrading bacteria, which can be isolated from cellulose enriched environments, were isolated with carboxymethylcellulose sodium （CMC-Na） as sole carbon source, CMC-Congo red staining method was used to further screen, and one strain with larger transparent circle was obtained, named as B 1. Through the identification of bacterial colony, cell morphology observation, physiology and biochemistry experiment, and molecular biology, initially identified as Bacillus cereus, the strain showed a high ability of disintegration of filter paper and higher cellulase activity. Through the optimization of culture conditions, it was found that the strain was in the culture medium with the initial pH value of 5.0, temperatre 37℃ and after 36 hours, the cellulase activity could reach 64.22 U/mL.