摘要
活体细胞体内代谢途径调控机制复杂,若能将目的代谢途径移到胞外,则能进行直观研究。选用嗜热酶来源的10条基因,构建一个能实现自我能量ATP和辅酶NADP(H)循环平衡,葡萄糖代谢生成乳酸的体外合成途径。对该途径初步研究表明,最适反应p H7.0,最适反应温度50℃,该条件下添加少量的辅酶NADP+,8 h内能将12.4 g的葡萄糖转化生成9.5 g的乳酸。
The regulating circles of the metabolic pathways in cells in vivo are complicated, and the intuitive researches may be carried out if the target metabolic pathways could be moved to the cells in vitro. Selecting 10 thermophilic enzymes, synthes＇izing pathways in vitro were constructed, which achieved the cycle balance of energy ATP and redox cofactors NAHP （ H ）, as well as the produced lactate from glucose metabolism. The preliminary study revealed this system＇ s optimal reaction condition was pH 7 and 50℃. Under this condition, adding small amount of NADP＋, 12.4 g/L glucose was converted to 9.5 g/L lactate within 8 hours.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第9期260-266,共7页
Biotechnology Bulletin
基金
国家重点基础研究发展计划("973"计划)(2011CBA00804)
关键词
体外合成
代谢途径
基因工程
多酶催化体系
synthesis in vitro
metabolic pathway
genetic engineering
multi-enzyme synthesis
