Box-Behnken设计优化甘草浸膏中甘草苷的超声提取

Optimization of ultrasonic extraction of liquiritin from licorice extract by Box-Behnken design

以新疆甘草浸膏为原料,利用Box-Behnken设计优化超声提取甘草浸膏中甘草苷的工艺。在单因素试验的基础上,利用响应面法考察固液比、超声时间和乙醇体积分数对甘草苷含量的影响,优化提取工艺,得到最佳工艺条件:固液比2.5 g/L、超声时间24 min、乙醇体积分数59%,甘草苷一次提取含量达到0.832%。此研究对甘草浸膏的综合利用以及产业化发展提供了科学依据。

Single factor test and Box？Behnken design method were adopted to optimize an ultrasonic-assisted extraction process of liquiritin from Xinjiang licorice extract. Three factors including ratio of material to liquid, supersonic time, ethanol concentration were selected with Box-Behnken design. The optimal conditions were：ratio of material to liquid 2. 5 g/L,ultrasonic time 24 min,ethanol volume ratio 59%.Under these conditions,the extraction rate of liquiritin could reach 0. 832% at one-time extraction. This research might provide the basis for the comprehensive utilization and industrialization of licorice extract.