摘要
为获得对核桃根腐病具有优良防治效果的解淀粉芽孢杆菌BA-12可湿性粉剂,采用单因素法和正交试验法筛选出对菌株BA-12活性影响最小和悬浮率最佳的载体和助剂及其配比。可湿性粉剂的配方为解淀粉芽孢杆菌10%,载体高岭土30%,湿润剂十二烷基苯磺酸钠10%、分散剂三聚磷酸钠5%、保护剂腐殖酸2.5%。主要性能检测结果表明可湿性粉剂活菌含量为1×10~8 cfu/g,湿润时间为40 s,悬浮率为79%,pH 6.72,含水率2.61%。稳定性测定中,菌剂对温度、酸碱、紫外光耐受性强,30~45℃、p H 6~8、紫外照射20~30 min均对菌剂影响小。盆栽试验得到500~1000倍稀释的菌剂为防治核桃根腐病推荐使用浓度。
The single factor and orthogonal test methods were used to screen the carriers, additives and their proportioning to obtain Bacillus amyloliquefaciens BA-12 wettable powder with excellent control effect on walnut root rot. The B. amyloliquefaciens BA-12 wettable powder prescription were comprised 10% B. amyloliquefaciens, 30% the carrier kaolin, 10% sodium dodecyl benzene sulfonate, 5% dispersing agent sodium tripolyphosphate, 2.5% protecting agent humic acid, with the content of living bacterium was 1×108 cfu/g. Its wetting time was 40 s, suspension rate was 79%, p H 6.72, rate of water content was 2.61%. The B. amyloliquefaciens BA-12 wettable powder were stable under the conditions of 30―45 ℃, p H 6―8 and ultraviolet irradiation 20―30 min. The 500―1000 times diluents on walnut root rot was the recommended concentration in potted tests.
作者
李姝江
方馨玫
曾艳玲
樊苏恒
朱天辉
LI Shujiang FANG Xinmei ZENG Yanling FAN Suheng ZHU Tianhui(College of Forestry, Sichuan Agricultural University, Chengdu 611130, China)
出处
《中国生物防治学报》
CSCD
北大核心
2016年第5期619-626,共8页
Chinese Journal of Biological Control
基金
基金项目:国家自然科技资源共享平台(2005DKA21207-13)