稳定性大鼠脊髓损伤动物模型的复制及评价

Replication and evaluation of rat model with stable spinal cord injury

目的建立一种实用、标准、可靠的急性大鼠脊髓损伤（SCI）模型，并对其稳定性进行评价。方法将20只SD大鼠随机均分为SCI组和对照组。SCI组打开T8～T10节段脊髓，暴露硬脊膜，在T9水平用Leica Impact One打击器打击，力度设定为0．5m／s，以鼠尾痉挛性摆动并下垂、双下肢软瘫为模型成功标准；对照组只暴露硬脊膜。分别于造模前及造模后各时间点进行两组双后肢BBB运动功能评分。结果造模成功率100％。SCI组于造模第1周开始恢复，观察至造模后第4周基本停止，造模后各时间点SCI组大鼠BBB运动功能评分均低于对照组（P〈0．05）。脊髓HE染色及电镜结果符合SCI的病理学变化，且死亡率相对较低。结论采用Leica Impact One打击器建立的急性SCI模型具有建模方法简便、稳定性高、死亡率低的优点。

Objective To establish a rat model with stable spinal cord injury and evaluate its stability. Methods Twenty SD rats were equally randomized into two groups of A and B. The rat spinal cord in group A was opened at T8-T10 segments and then spinal dural mater was exposed. The T9 segment was hit by Leica Impact One Strike in a speed of 0.5 m/s. When ptosis and spastic swing the tail appeared and double limbs were paralyzed, the model was successfully established. The rat spinal dural mater in group B was exposed only as the control. The locomotors function was evaluated by Basso-Beattie-Bresnahan （BBB） score in two groups before and after modeling. Results The modeling success rate was 100%. The spinal cord injury in group A began recovering in the first week after modeling, and then basically stopped in the fourth week after modeling. The BBB locomotors scores at each time point after modeling were less in group A than those in group B（P〈0.05）. The pathological changes in spinal HE staining and electron microscopy were consistent with the spinal cord injury,and the mortality rate was relatively lower. Conclusion The establishment of acute spinal cord injury rat model using Leica Impact One Strike has the advantages of simple modeling method, high stability and low mortality.