miR-205对卵巢癌细胞SKOV-3侵袭迁移力的影响

Effects of miR-205 on migration and invasion of ovarian cancer SKOV-3 cells

目的研究微小RNA-205(miR-205)在卵巢癌细胞株中的表达,探讨miR-205对人卵巢癌细胞SKOV-3迁移和侵袭能力的影响及其可能机制。方法通过实时荧光定量PCR(qRT-PCR)方法检测3种卵巢癌细胞株(SKOV3,A2780,OVCAR-3)和正常卵巢细胞株(IOSE80)中miR-205的表达,利用miR-205 mimic瞬时转染SKOV3细胞,qRT-PCR检测转染后细胞内的miR-205表达水平,采用划痕试验检测SKOV3细胞的迁移能力,Transwell试验检测SKOV3细胞的侵袭能力,Western blot实验检测细胞内E-cadherin、N-cadherin及Vimentin蛋白表达水平。结果 miR-205在人卵巢癌细胞株中的表达较正常卵巢细胞高,在SKOV3细胞中转染miR-205 mimic,miR-205表达明显升高(t=8.841,P<0.01),miR-205 mimic可增强卵巢癌细胞SKOV-3的迁移能力和侵袭能力,促进细胞内E-cadherin蛋白表达。结论 miR-205在人卵巢癌细胞中高表达,过表达miR-205可促进卵巢癌细胞SKOV-3的侵袭转移,可能成为干预卵巢癌转移复发的分子靶点。

Objective To investigate the expression of microRNA-205 （miR-205） in ovarian cancer cell strains, the impact of miR-205 on migration and invasion of ovarian cancer SKOV-3 cell. Methods Real-time quantitative PCR （qRT-PCR） was applied to detect the expression of miR-205 in 3 kinds of ovarian cancer cell strains （SKOV3, A2780, OVCAR-3） and normal ovarian cell line （IOSE80）. miR-205 mimic was transiently transfected into SKOV3 cells, and then the expression of miR-205 was detected by qRT-PCR. Wound healing assay and transwell assay was used to detect the migration and invasion ability of SKOV3 cells. The expression levels of E-cadherin, N-cadherin and Vimentin in cells were detected by Western blot. Results MiR-205 was over-expressed in ovarian cancer cell strains as compared with normal ovarian ceils. MiR-205 mimic significantly increased the expression of miR-205 in SKOV3 cells （t=8.841, P〈0.01）. MiR- 205 mimic can enhance the migration and invasion of ovarian cancer cell SKOV-3 and promote the expression of E- cadherin protein in cells. Conclusion MiR-205 is highly expressed in ovarian cancer cells. Over expression of miR-205 may promote the invasion and metastasis of ovarian cancer cell line SKOV-3, which may be a molecular target for the intervention of ovarian cancer metastasis and recurrence.