藏药蕨麻对原代培养小鼠肝细胞酒精损伤保护作用研究

Protective Effect of Tibetan Medicine Potentilla Anserina L. on Ethanol-induced Damage in Prime Liver Cell of Mice

目的研究藏药蕨麻对原代培养酒精损伤肝细胞的保护作用。方法原代肝细胞经分离纯化培养后,MTT法评价藏药蕨麻对酒精损伤肝细胞存活率的影响;荧光染色法测定藏药蕨麻对酒精损伤肝细胞活性氧物质(ROS)含量、细胞内钙离子浓度的影响;流式细胞仪检测藏药蕨麻对酒精损伤肝细胞凋亡的影响;免疫印迹法检测藏药蕨麻对Bcl-2和Bax表达的影响。结果经酒精损伤后,肝细胞存活率降低;细胞内ROS含量和钙离子浓度增高;凋亡抑制基因Bcl-2减弱、促凋亡基因Bax表达增强。藏药蕨麻可明显提高细胞存活率;降低细胞内ROS含量和钙离子浓度;改善凋亡情况,增强Bcl-2表达,抑制Bax表达,且作用呈剂量依赖性。结论藏药蕨麻对原代培养小鼠肝细胞酒精损伤具有一定的保护作用。

OBJECTIVE To study the protective effct of Tibetan medicine Potentilla anserina L. on ethanol-induced damage in mice prime liver cell. METHODS After isolation, purification and culture of primary hepatocytes, the livability of cell was evaluated by MTT; fluorescence microscopy were used to evaluated the contents of reactive oxygen species（ROS） and concentration of Ca^2＋; flow cytometry was used to detect the cell apoptosis; the expression of Bcl-2 and Bax were detected by Western bloting. RESULTS After damaged by alcohol, the cell livability decreased, the contents of Ros and concentration of Ca^2＋ increased, the expression of Bcl-2 decreased while that of Bax increased. Potentilla anserina could increase the cell livability, decreased the intracellular ROS content and Ca^2＋ concentration ameliorate the apoptosis, increase the expression of Bcl-2, and decrease the expression of Bax. CONCLUSION Tibetan medicine Potentilla Anserina L. has protective effect on ethanol-induced damage in prime liver cell of mice.