摘要
研究了3种微生态制剂枯草芽孢杆菌(A)、粪链球菌(B)、光合细菌(C)两两配伍,以105CFU/ml的浓度按9∶1、4∶1、1∶1、1∶4、1∶9混合制成15种制剂,对池塘水质中COD、氨态氮、亚硝酸盐氮、硫化物的影响。结果表明,以1∶4混合制成的枯草芽孢杆菌和光合细菌的混合制剂对COD的处理效果最好(P<0.05),以4∶1混合制成的枯草芽孢杆菌和粪链球菌的混合制剂对氨态氮的处理效果最好,对亚硝酸盐氮处理效果最好的是以4∶1配制的粪链球菌和光合细菌的混合制剂(P<0.05),对硫化物的处理效果最好是以9:1混合制成的粪链球菌和光合细菌的混合制剂(P<0.05)。
In order to provide reference for probiotics application in aquaculture, Bacillus subtilis (A), Streptococcus faecalis (B) and photosynthetic bacteria (C) were prepared according to the ratios of 9:1, 4:1, 1:1, 1:4 and 1:9 with 10^5 cfu/ml as the unit of concentration into 15 mixed microecological preparations, and their effects on COD, ammonia nitrogen, nitrite nitrogen and sulfide in pond water were investigated. The results showed that the mixed preparation of B. subtilis and photosynthetic bacteria at a ratio of 1:4 had the best effect in treating COD (P〈0.05), the mixed preparation of B. subtilis and S. faecalis at a ratio of 4:1 showed the best effect in treating ammonia nitrogen (P〈0.05), the mixed preparation of S. faecalis and photosynthetic bacteria at a ratio of 4:1 showed the best effect in treating nitrite nitrogen (P〈0.05), and the mixed preparation of Streptococcus faecalis and photosynthetic bacteria at a ratio of 9:1 had the best effect of reducing sulfide (P〈0.05).
基金
Supported by Science and Technology Planning Project of Hunan Province(2012NK3097)
Fund for Key Discipline Construction(Zoology)of Hunan Province during the"12thFive-Year Plan"(2015-007)
Open Fund of Hunan Province Key Laboratory of Health Aquaculture and Processing(2015-011)
Science and Technology Innovation Team Plan of Hunan Provincial Colleges and Universities(2014-031)~~
关键词
复合微生态制剂
枯草芽孢杆菌
光合细菌
粪链球菌
水质净化
Compound microecologicat preparation
Bacillus subtilis
Photosynthetic bacteria
Strptococcus faecalis
Water purification