响应面法优化超临界二氧化碳萃取法提取姜精油的工艺（英文）

Optimization of Supercritical Carbon dioxide Extraction of Ginger Essential Oil by Response Surface Method

[目的]建立从生姜中获得高质量和高纯度姜精油的提取方法。[方法]应用超临界流体萃取技术,采取单因素和响应面优化方法,选取二氧化碳流量、姜粉过筛目数和夹带剂加入量三个因素作为考察指标,以6-姜酚得率作为姜精油提取的考察指标。[结果]通过响应面实验分析结果得到,最优姜精油提取条件为:二氧化碳流量为25 L/h,姜粉过筛目数为80目,夹带剂加入量为加入无水乙醇92.46 m L,6-姜酚的最优得率为3.21%。[结论]采用超临界二氧化碳萃取技术,通过单因素和响应面法优化实验,实现了从生姜中分离提取出高质量和高纯度姜精油的目标。

[Objective] Ginger essential oil （GEO） is widely used in food production and medical field in recent years due to its prominent biological functions, and this study was conducted to obtain high-quality and high-purity ginger essential oil from the fresh ginger. [Method] GEO was extracted from ginger roots by supercritical fluid extraction （SFE） method. The effects of flow rate of CO2, mesh size of ginger powder and volume of entrainer were investigated by single-factor experiments and response surface method. The content and extraction rate of 6-gingerol represented the extraction index of GEO. [Result] The conditions were optimized as follows： flow rate of CO2 at 25 L/h, mesh size of ginger power of 80 mesh, and volume of anhydrous ethanol as entrainer of 92.46 ml. The optimal extraction rate of 6-gingerol was 3.21%, which was predicted by RSM. [Conclusion] The optimal process of supercritical carbon dioxide extraction of ginger essential oil was identified by singlefactor experiments and response surface method. The present study provides a satisfactory method for purifying GEO from ginger for industrial purpose.