肉牛黄体期和卵泡期阴道菌群结构的比较研究

Comparison of vaginal micro flora structure of beef cattle between luteal and follicular phase

为了研究生殖激素对阴道菌群的影响,选取7头健康肉牛,分别采集黄体期和卵泡期阴道分泌物,提取细菌总DNA,根据DGGE克隆测序结果,选择5种特异性细菌,利用荧光定量PCR技术检测各细菌数量。结果表明,总菌、大肠杆菌、鞘氨醇单胞菌和睡眠嗜血杆菌菌群数量在两组间无显著差异(P>0.05),但黄体期链球菌属数量显著高于卵泡期(P<0.01),卵泡期乳杆菌属数量显著高于黄体期(P<0.05)。结果提示,肉牛黄体期与卵泡期的阴道菌群结构存在显著差异。

In order to investigate the effect of hormones on the vaginal flora, vaginal swab samples from seven cattle were col- lected during the luteal and follicular phase .Total bacterial DNA was extracted for real-time PCR. Following the DGGE cloned re- sults, five specific bacteria species were selected. The real-time PCR was used to measure the quantity of the vaginal flora between the luteal and follicular phase, respectively. The results indicated that there was no significant difference between the luteal phase and follicular phase in total bacteria species, Escherichia coli, Sphingomonas and Haemophilus somnus （P〉0.05）. However, the Streptococcus number was significantly higher in the luteal phase than that in follicular phase （P〈0.01）. Conversely, follicular phase Lactobacillus was significantly higher than that in the luteal phase （P〈0.05）. The current results suggest that beef cattle mi- cro flora structure differs significantly during the luteal phase and the follicular phase.