中哈边境艾比湖湿地游离蜱斑点热群立克次体的分子流行病学研究

Molecular epidemiological study of the spotted fever group Rickettsia in free-living ticks in the wetlands of Aibi Lake,China-Kazakhstan border

了解中哈边境新疆艾比湖湿地游离蜱中斑点热群立克次体的感染情况。利用斑点热群立克次体外膜蛋白A(ompA)基因对艾比湖湿地游离亚洲璃眼蜱、边缘革蜱、血红扇头蜱进行PCR检测,并对阳性样本进行测序和BLAST序列分析,并应用Mega5.0软件建立分子系统进化树。结果中哈边境地区艾比湖湿地游离蜱斑点热群立克次体电泳阳性率36.84%(56/152),BLAST分析显示,艾比湖湿地游离蜱斑点热群立克次体基因型为Rickettsia raoultii,与从匈牙利患蜱传染的病人肿大淋巴结中分离获得的R.raoultii关系最近,处于同一分支(Gen Bank登录号为JQ798904)。结论是新疆艾比湖湿地游离蜱斑点热立克次体感染较为严重,存在立克次体自然疫源地,应尽快制定防制措施,以免危机动物及人类健康。

To investigate the prevalence of tick-borne spotted fever group Rickettsia（SFGR）in the wetlands of Aibi Lake,China-Kazakhstan border and to provide a scientific basis for the prevention and control of tick-borne SFGR.The Omp A gene am-pliflcation was carried out for DNA samples extracted from Hyalomma asiaticum,Dermacentor marginatus,and Rhipicephalus sanguineus collected in the wetlands of Aibi Lake,China-Kazakhstan border and the positive samples were subjected to sequencingand sequence analysis to establish the molecular phylogenetic tree.56（36.84%）of the 152 DNA samples were positive for SFGR.The phylogenetic analysis based on nucleotide sequence showed that all detected SFGR strains were clustered together with（acces-sionnumbers：JQ798904）;These strains had the highest homology with Rickettsia raoultii（99.02%）.Tick-borne SFGR is prevalentin the wetlands of Aibi Lake on China-Kazakhstan border.Preventive measures should be taken in time to protect humans and ani-mals from this disease.