延边某地长角血蜱和全沟硬蜱体内东方泰勒虫的分子生物学检测

The Molecular detection of Theileria orientalisi in Haemaphysalis longicornis and Ixodes persulcatus in Yanbian area

为调查吉林省延边地区自然生境内游离蜱携带东方泰勒虫的情况,以东方泰勒虫主要表面蛋白基因(MPSP)基因为靶基因,采用PCR方法对延边地区自然生境内的游离蜱DNA进行扩增。在长角血蜱的DNA中检测了东方泰勒虫,检出率为29%;而全沟硬蜱中没有检测到东方泰勒虫。结果表明,自然生境中的游离长角血蜱体内携带着东方泰勒虫。

In order to investigate the existence of Theileria orientalisi in free ticks in Yanbian area of Jilin province,the polymerase chain reaction（PCR）method was used to amplify major piroplasm surface protein（MPSP）gene of T.orientalisi. The MPSP gene was detected in Haemaphysalis longicornis DNA,and the detection rate was 29%. However,the MPSP gene was not detected in Ixodes persulcatus DNA samples. The results showed that the free H. longicornis in the natural habitat of Yanbian carried the T.orientalisi.