益生菌干预对芽囊原虫感染大鼠血清IL-4、IL-10、IL-12和IL-17的影响

Impact of Probiotics on the Levels of Serum Cytokine IL-4,IL-10,IL-12and IL-17 after Blastocystis hominis Infection in Rats

目的:研究益生菌干预对芽囊原虫感染大鼠外周血IL-4、IL-10、IL-12和IL-17的影响,探讨益生菌、芽囊原虫感染和肠道菌群失调三者的关系。方法:48只雄性SD大鼠按三因素(益生菌干预、芽囊原虫感染和肠道菌群失调)两水平(0、1)随机分为8组。取芽囊原虫包囊液103·只-1灌胃,连续5 d,建立感染模型。用盐酸克林霉素以5 000 mg·kg-1灌胃,连续5 d,建立肠道菌群失调模型。芽囊原虫感染合并菌群失调模型在感染模型建立后用盐酸克林霉素建立。建模后1 d,金双歧2 g·d-1经口饲喂,共7 d。ELISA法检测大鼠血清IL-4、IL-10、IL-12和IL-17水平。结果:益生菌干预、芽囊原虫感染对IL-4和IL-10水平的影响均有统计学意义(P<0.05),且益生菌干预与芽囊原虫感染之间存在交互作用(P<0.05)。益生菌干预、菌群失调对IL-12水平的影响有统计学意义(P<0.05),益生菌干预与芽囊原虫感染,益生菌干预与菌群失调之间存在交互作用(P<0.05)。各组间IL-17水平未见显著性差异(P>0.05)。结论:芽囊原虫感染增高大鼠外周血IL-4、IL-10水平,菌群失调增高IL-12水平,合并芽囊虫感染IL-12水平较之降低,益生菌干预可改善这些细胞因子的水平。

Objective： To investigate the impact of probiotics on the levels of serum cytokine IL-4、IL-10、IL-12 and IL-17 after Blastocystis hominis infection in rats,and to explore the interaction among probiotics,B. hominis infection,and intestinal dysbiosis. Methods： Fourty-eight SD rats were randomly assigned to 8 groups according to factorial design with three factors（ probiotics,B. hominis infection,and intestinal dysbiosis） and two levels（ 0 and 1）. The rats in infection groups orally received 10^3/ day B. hominis cysts for 5 days. The rats in intestinal dysbiosis groups were orally given 5 000mg·kg^- 1lincomycin hydrochloride for 5 days. The rats in mix model groups were infected with B. hominis at first 5 days and then were fed with lincomycin hydrochloride for another 5 days. The probiotics groups were fed with 2 g·d^- 1golden bifidobacteriums tablets for 7 days after mix models were set up. The levels of serum cytokine IL-4,IL-10,IL-12,and IL-17 were detected by ELISA. Results： Both probiotics and B. hominis infection significantly affect the levels of serum cytokine IL-4 and IL-10（ P〈0. 05）,and there is interaction between probiotics and B. hominis infection（ P〈0. 05）. Both probiotics and intestinal dysbiosis have significant impact on the level of serum cytokine IL-12（ P〈0. 05）,and there is interaction between probiotics and intestinal dysbiosis（ P〈0. 05）. There is no significant difference in serum IL-17 level among all groups（ P〉0. 05）. Conclusion： B. hominis infection results in increased serum IL-4 and IL-10 levels,and intestinal dysbiosis lead to increased serum IL-12 level. However,B. hominis infection could counteract the effect of intestinal dysbiosis in terms of IL-12 level and probiotics could balance the levels of cytokine IL-4,IL-10,and IL-12.