棉花黄萎病抗性鉴定及抗病相关的分子标记检测

Identification of Cotton Resistance to Verticillium wilt and Detection of Correlation Molecular Maker of Resistance

以20个不同黄萎病抗性的海岛棉(G.barbadense)、陆地棉(G.hirsutum)品种为材料,使用11对与抗黄萎病基因连锁的SSR多态性引物对其进行SSR-PCR扩增分析,并计算各抗病品种扩增条带与抗性性状的一致程度。结果筛选到VM15、VM18、VM22、VM23和VM32共5对分子标记能够获得特异性产物,该标记是根据抗病相关的mRNA序列、Protein基因序列和基因组序列设计合成的。计算可得引物VM32、VM15、VM23获得的特异性产物与棉株抗病性一致率分别是1.000、0.857、0.857,均高于0.800,获得的6条特异性条带含有目的抗性基因。

G.barbadense,G.hirsutum with 20 varying degree of resistance to Verticillium wilt and 11 SSR polymorphisms primers were used to conduct SSR-PCR amplification analysis and the consistant degree of the disease-resistant cultivars amplified bands and the resistance properties.It was found that 5pairs of molecular maker such as VM15,VM18,VM22,VM23,VM32 were screened,which could obtain peculiar products,the makers were composed of mRNA sequence,protein gene sequence and genome sequence.The identical rate of the peculiar product and cotton plant calculated from VM32,VM15,VM23 resistance were1.000,0.857,0.857 respectively,the average were higher than 0.800.So the six specific bands contained resistance genes.