氧化石墨烯负载多柔比星对多发性骨髓瘤细胞的杀伤作用

Graphene oxide loaded with doxorubicin: a killer for multiple myeloma cells

背景:研究发现氧化石墨烯对正常细胞的毒性比较低,但有关氧化石墨烯负载多柔比星抗肿瘤药物后对恶性肿瘤细胞是否有影响的研究不多。目的:探讨氧化石墨烯负载多柔比星对多发性骨髓瘤细胞的杀伤作用。方法:取对数生长期人多发性骨髓瘤细胞株RPMI8226,分4组培养,其中3组分别加入氧化石墨烯负载多柔比星、多柔比星、氧化石墨烯,对照组不干预。培养24 h,CCK-8方法检测细胞活性,流式细胞仪检测细胞周期和细胞凋亡。结果与结论:(1)对照组细胞质透亮清晰,多数为饱满形态;氧化石墨烯组细胞质较透亮,少数细胞出现皱缩;多柔比星组细胞形态不均一,细胞出现皱缩,有凋亡小体出现;氧化石墨烯负载多柔比星组细胞体积明显缩小,皱缩较明显,出现凋亡小体;(2)氧化石墨烯负载多柔比星组、多柔比星组、氧化石墨烯组细胞存活率均低于对照组(P<0.05),氧化石墨烯负载多柔比星组细胞存活率明显低于氧化石墨烯组(P<0.05);(3)氧化石墨烯负载多柔比星组、多柔比星组细胞凋亡率高于氧化石墨烯组、对照组(P<0.05);(4)4组间细胞周期比较差异无显著性意义;(5)结果表明,氧化石墨烯负载多柔比星具有较强的毒性作用,可诱导人多发性骨髓瘤细胞凋亡。

BACKGROUND： Cytotoxicity of graphene oxide to normal cells is relatively low, but whether graphene oxide loaded with doxorubicin has some effects on malignant cells is rarely reported. OBJECTIVE： To explore the cytotoxicity of graphene oxide loaded with doxorubicin on multiple myeloma cells. METHODS： Multiple myeloma cell line RPMI8226 in logarithmic phase was selected, cultured and divided into four groups, including graphene oxide loaded with doxorubicin, doxorubicin and graphene oxide groups as well as control group with no intervention. After 24 hours of culture, the cell activity was detected by cell counting kit-8 method, and the cell cycle and apoptosis were detected using flow cytometry. RESULTS AND CONCLUSION： Plump-shaped cells with translucent and clear cytoplasm were found in the control group; cells with relatively translucent cytoplasm, and even a few shrunken cells appeared in the graphene oxide group; cellular morphology was in a heterogeneity, apoptotic bodies appeared in the doxorubicin group; the cells was significantly reduced in size, presenting more obvious shrinkage and apoptotic bodies in the group of graphene oxide loaded with doxorubicin. The cell survival rate in the graphene oxide loaded with doxorubicin, doxorubicin and graphene oxide groups was significantly lower than that in the control group（P 〈0.05）, and this indicator was significantly lower in the group of graphene oxide loaded with doxorubicin than the graphene oxide group（P 〈0.05）. The apoptotic rate in the group of graphene oxide loaded with doxorubicin and doxorubicin group was significantly higher than those in the graphene oxide and control groups（P〈 0.05）, respectively. Additionally, there were no significant differences in the cell cycle among groups. These results show that graphene oxide loaded with doxorubicin has a stronger cytotoxicity, and can induce apoptosis in human multiple myeloma cells.