ZnS量子点荧光分光光度法检测牛血清白蛋白含量的研究

Determination of bovine serum albumin by ZnS quantum dot fluorometry

通过水热法合成水溶性ZnS量子点（QDs）,透射电子显微镜和X-粉末衍射仪检测的结果显示其粒径在6-8 nm。荧光分析表明该量子点有良好的荧光稳定性。牛血清白蛋白（BSA）可以增强ZnS QDs的荧光强度,在一定浓度范围内ZnS QDs的荧光增强与BSA的浓度呈正相关,通过不同浓度BSA影响ZnS QDs荧光强度的变化,实现BSA含量的检测。通过检测条件优化,蛋白浓度在0-160 mg/L条件下荧光强度与BSA浓度之间良好的线性关系,线性方程为y=45.7685x＋351.70,相关系数R^2=0.9986,验证试验表明荧光光度法测定BSA具有一定的可重复性与准确度,对蛋白含量的快速检测有潜在的应用价值。

Water-soluble Zn S quantum dots（ QDs） have been prepared via a facile hydrothermal method.The resulting Zn S QDs had a spherical morphology and size in the range of 6- 8 nm,as characterized by X-ray diffraction and transmission electron microscopy.The optical properties were investigated by photoluminescence spectroscopy,which showed that the fluorescence intensity was stable.Based on the increase in fluorescence intensity in the presence of bovine serum albumin（ BSA）,a method for the determination of the protein was established.It was found that the fluorescence intensities had a good linear relationship with the concentration of BSA in the ranges 0- 160 mg/L.The linear equation was y = 45.7685 x ＋ 351.70 and the correlation coefficient（ R^2） was 0.9986.Zn S QDs fluorometry thus shows potential applications in protein concentration determination.