石灰、蒌叶处理槟榔对小鼠生殖毒性及体温的影响

Influence of Lime,Piper betle Processing Betelnut on Mice'Genital Toxicity and Body Temperature

为探索添加蒌叶和石灰对槟榔毒副作用的影响,设置蒌叶+槟榔、石灰+槟榔、蒌叶+石灰+槟榔、槟榔4个处理及空白对照(蒸馏水),分别将不同处理的水提液(蒸馏水)对SPF级KM小鼠进行灌胃处理,每天1次,连续灌胃14 d,测定小鼠的生精细胞凋亡指数、Bcl-2与Bax蛋白表达量及体温。结果表明,与空白对照相比,各处理的睾丸曲细精管中生精细胞凋亡指数均增高且差异显著,其中槟榔处理的凋亡指数最大,其次是槟榔+石灰,槟榔+蒌叶+石灰处理最小,槟榔处理的凋亡指数是槟榔+蒌叶+石灰处理的2.6倍;各处理的Bax蛋白表达阳性率均增高,Bcl-2蛋白表达阳性率均降低,其中槟榔处理变化幅度最大,槟榔+蒌叶+石灰槟榔处理最小,槟榔+蒌叶+石灰处理的Bcl-2/Bax值是槟榔处理的1.41倍;各处理的小鼠体温均呈现下降趋势,槟榔+蒌叶+石灰处理体温相对最高且降幅最小,与槟榔处理相反。可见,添加蒌叶或石灰能减弱槟榔对小鼠的毒性作用。

To explore the toxic effects of betelnut after Piper betle and lime were added,the KM mice of SPF were intragastricly administered with water extract solution from Piper betle ＋ betelnut,lime ＋ betelnut,Piper betle ＋ lime ＋ betelnut and betelnut once a day and observed for 14 d. Spermatogenic cell index,Bcl-2,Bax and body temperature of mice were measured. The results showed that spermatogenic cell apoptosis index of treatments were increased and had a significant difference with the control,in which the betelnut treatment was the biggest and was 2. 6 times of Piper betle ＋ lime ＋ betelnut treatment that was the smallest,and lime ＋ betelnut was between them. The positive expression level of Bax protein of the treatments were up regulated,but Bcl-2 protein was down regulated significantly,which betelnut treatment changed was the biggest,and Piper betle ＋ lime ＋ betelnut was minimum,but the Bcl-2 / Bax of the laterwas 1. 41 times of the former. Body temperature of mice in four treatments were lower than CK and showed a trend of decline,Piper betle ＋ lime ＋ betelnut treatment ＇s temperature was relatively higher and the smallest decrease,in contrast with betelnut. In short,adding Piper betle or lime to betelnut could weaken genital toxicity in mice.