硫化氢对臭氧致小鼠气道炎症的影响

Effect of hydrogen sulfide on airway inflammation induced by ozone in mice

目的:研究硫化氢(H_2S)对臭氧(O_3)暴露所致小鼠气道炎症的影响,并探究其机制。方法:32只C57BL/6小鼠随机分为正常对照(control)组、O_3组、硫氢化钠(NaHS)+O_3组以及NaHS组。第1、3、5天将O_3组和NaHS+O_3组小鼠置于2.14 mg/m^3O_3环境中暴露3 h,同时control组和NaHS组置于新鲜空气中暴露3 h。每次暴露前30 min,NaHS+O_3组和Na HS组小鼠腹腔注射Na HS(14μmol/kg)。末次暴露24 h后测定小鼠气道反应性,收集支气管肺泡灌洗液用于炎性细胞计数和总蛋白测定,收集肺组织标本行HE染色观察形态改变。测定肺组织中IL-6、IL-8、丙二醛(MDA)和NF-κB p65蛋白水平。结果:与control组相比,O_3组的气道反应性、炎性细胞数、总蛋白浓度和炎症评分,以及IL-6、IL-8、MDA和NF-κB p65蛋白水平明显增高,NaHS+O_3组则明显低于O_3组。结论:H_2S显著减轻了O_3暴露所致气道炎症反应,可能与抑制脂质过氧化和降低NF-κB表达相关。

AIM：To investigate the effect of hydrogen sulfide （ H2 S） on airway inflammation induced by ozone （O3） exposure and its mechanisms.METHODS：C57BL/6 mice （n=32） were randomly divided into control group, O3 group, NaHS＋O3 group and NaHS group.The mice in O3 group and O3 ＋NaHS group were exposed to 2.14 mg/m3 O3 for 3 h on days 1, 3 and 5, while the mice in control group and NaHS group were exposed to filtered air .NaHS （14μmol/kg） was administered intraperitoneally to the mice in NaHS group and O 3 ＋NaHS group 30 min before each exposure .After the last exposure for 24 h, the airway responsiveness was determined , and bronchoalveolar lavage fluid （ BALF） was collected for counting inflammatory cells and measuring total protein concentration .The lung tissues were collected for observing the morphological changes with HE staining .The levels of interleukin-6 （ IL-6 ） , interleukin-8 （ IL-8 ） , malondialdehyde （ MDA） and NF-κB p65 protein in the lungs were determined .RESULTS： Compared with control group , the airway re-sponsiveness, inflammatory cells, protein concentration, inflammation score, levels of IL-6, IL-8, MDA and NF-κB p65 in O3 group increased significantly , but these in NaHS＋O3 group decreased compared with O 3 group.CONCLUSION： The present findings suggest that H 2 S attenuates O3 induced airway inflammation by inhibiting NF-κB expression and preventing lipid peroxidation .