摘要
在已有测序数据基础上,利用三种常见的序列组装软件对Paenibacillus Shenyangensis全基因组测序结果进行拼接组装,分析比较了不同软件在各自最优参数条件下DNA序列的组装数据,并与NCBI数据库中类芽孢杆菌属其他近缘种进行基因比对与预测.结果表明,SOAPdenovo的组装结果最优,在k-mer为23时,组装基因组总长和N50分别为5 501 467和293 864 bp,预测的4 800个基因中有4 393个与NCBI-Nr数据库比对并注释成功.
On the basis of existing sequencing data,three sequence assembling programs were utilized to assemble the genome sequence of Paenibacillus Shenyangensis. The assembly data of DNA sequence were analyzed and compared with different software in their optimal parameters,and were compared with genes of the other species of the Paenibacillus in the NCBI database. The results showed that SOAPdenovo is the most appropriate assemble software. When k-mer is 23,the total genome length and N50 are 5 501 467 and 293 864 bp,respectively. 4 393 of the total4 800 genes are successfully matched and annotated.
作者
付丽丽
姜彬慧
胡筱敏
FU Li- li JIANG Bin- hui HU Xiao-min(School of Resources ＆ Civil Engineering, Northeastern University, Shenyang 110819, China College of Petroleum Engineering, Liaoning Shihua University, Fushun 113001, China.)
出处
《东北大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2016年第10期1465-1468,共4页
Journal of Northeastern University(Natural Science)
基金
国家科技重大专项(2013ZX07202-010)
国家自然科学基金资助项目(51278090)
关键词
序列拼接
基因组
絮凝菌
高通量测序
类芽孢杆菌
sequence assembly
genome
flocculants bacteria
high-throughput sequencing
Paenibacillus
