杨木MWL和LCC制备多孔生物载体及其在人体肝细胞培养中的应用

Preparation of Porous Biological Carrier with Aspen MWL and LCC and Application in Culture of Human Hepatocytes

为了比较磨木木质素(MWL)与木质素-碳水化合物复合体(LCC)与人体肝细胞的生物相容性,从杨木中提取MWL和LCC,制备水凝胶,冷冻干燥形成多孔生物载体。利用红外光谱和扫描电子显微镜对多孔生物载体的成分及表面形态进行了分析,采用多孔生物载体对人肝细胞进行体外培养,并对肝细胞形态及代谢活性进行检测。结果表明:LCC基载体中木质素含量较低而碳水化合物含量较高,因此其两亲性较MWL基载体好。以MWL和LCC制备的多孔生物载体,孔隙率达到60%左右,平均孔径约为30μm。细胞形态、白蛋白分泌和葡萄糖代谢的分析结果显示,相比MWL基载体,LCC基多孔生物载体能更好地促进肝细胞长期生长,具有更好的生物相容性。

In order to compare the biocompatibility of milled wood lignin （MWL） and lignin-carbohydrate complexes （ LCC ）, MWL and LCC were prepared with aspen wood in the present research.Hydrogels were prepared with these materials.Then ,porous biological carriers were obtained by freeze-drying. Fourier transform infrared spectrometer （FT-IR） and scanning eletron microscope （SEM） were applied to elucidate the composition and morphology of the porous biological carriers. Inverted microscope, albumin and glucose kits were used to monitor the morphology and metabolic activity of the cultured hepatocytes.The results indicated that LCC based carriers contained more carbohydrates and less lignin.Therefore, its amphipathicity is better than the MWL based carriers.The porosity of the MWL and LCC based carriers was about 60 % with average pore size of 30 μm.The results of analysis of cell growth, albumin and glucose metabolism showed that porous biological carriers prepared with aspen LCC could promote the growth of human hepatocytes during culture signficantly. Its biocompatibility is better than that of MWL.