HMGB1在蛛网膜下腔出血动物模型脑组织中的表达及其意义

Expression and Significance of HMGB1 in Brain Tissue of Animal Model of Subarachnoid Hemorrhage

目的通过蛛网膜下腔出血(SAH)动物模型观察高迁移率族蛋白B1(HMGB1)在SAH后脑实质和血清中的动态表达规律,以论证HMGB1在SAH炎性损伤中的作用。方法 SD大鼠视交叉池注血法制作SAH模型,动态观察SAH模型不同时间点血清、脑组织HMGB1水平以及脑组织病理变化,并与正常大鼠对照。结果视交叉池注血法制作SAH模型总体成活率约83.3%,模型稳定性较高。SAH动物模型血清中的HMGB1浓度与对照组差异无统计学意义(P>0.05)。免疫荧光显示SAH后脑组织内HMGB1阳性细胞明显高于对照组,蛋白印迹分析发现SAH大鼠脑组织HMGB1蛋白水平明显增高。结论 SAH大鼠脑组织HMGB1阳性细胞和蛋白水平明显增高,提示HMGB1介导的炎性信号通路可能在SAH后脑损伤中发挥着关键作用。

Objective To demonstrate the role of HMGB1 in the SAH inflammatory injury by subarachnoid hemorrhage （SAH）animal model to observe the high mobility group protein B1（HMGB1）in the brain parenchyma and the dynamic expression of SAH in serum. Methods We made SAH model by SD rat optic chiasmatical cistern blood injection method. The serum,brain tissue HMGB1 levels and brain tissue pathological changes of SAH modelat different time points were dynamically observed and were compared with normal rats. Results The general survival rate of SAH model made by prechiasmatic blood injection method is about 83. 3%,and the stability of the model is quite high. There was no significant difference between the HMGB1 concentration in the serum of SAH animal model and the control group（P〉0. 05）. Immunofluorescence staining showed that HMGB1 positive cells in the brain tissues were significantly higher than those in the control group. And the western blot analysis revealed that HMGB1 protein levels were significantly increased in SAH rats. Conclusion HMGB1 positive cells and protein levels were significantly increased in the brain of SAH rats,suggesting that the inflammatory signal pathway mediated by HMGB1 may play a key role in the brain injury after SAH.