煤系硅藻土-海藻酸钙协同固定化光合细菌去除废水氮磷实验

Experiment of Wastewater Treatment Using Immobilized Photosynthetic Bacteria by Synergistic Substrates of Coal-derived Diatomite and Alginate

以煤系硅藻土和海藻酸钙为复合载体材料,采用微流控方法,通过煤系硅藻土的亚微米连通多孔结构和海藻酸钙三维凝胶网络结构的协同作用,将光合细菌的沼泽红假单胞菌固定化,以提高固定化光合细菌去除废水氮磷的降解效能。选取化学需氧量(COD)、氨氮(NH_3-N)和总磷(TP)为污水的定量分析指标,探讨温度、光照强度、投放频率和投放量等环境因素对复合固定化光合细菌颗粒处理污水中氮磷的去除效能的影响。实验结果表明,煤系硅藻土-海藻酸钙协同固定化光合细菌去除废水氮磷的最优实验工艺条件为:温度30℃,光照强度为30×10~3lx,投加频率1/2 d^(-1),投加量20 g·L^(-1)。当复合固化颗粒处理废水6 d后,对废水氮磷的COD,NH_3-N和TP的去除率分别达到78.2%,78.7%和79.8%。

This paper demonstrated preparing immobilized photosynthetic bacteria of Rhodopseudomonas palustrisbeads using synergistic substrates of coal - derived diatomite and alginate by a microfluidic method. The synergistic structures between submicron - sized porous structure of diatomite and three - dimensional gel network structure of calcium alginate improved the removal efficiency of nitrogen and phosphorus in wastewater. The three parameters of chemical oxygen demand （COD） , ammonia nitrogen （NH3 -N） and total phosphorus （TP） were selected for quantitative analysis index. Environmental factors such as tempera-ture, light intensity, adding frequency and adding amount were discussed for obtaining optimized experimen-tal technology. The results show that the optimal experimental conditions are ： temperature of 30 ℃ , light intensity of 30×10^3 lx, adding frequency of 1/2 d1, andthe adding amount of 20 g·L^-1 with COD, NH3 - N and TPremoval rates of 78. 2% , 78. 7% and 79. 8% Respectively after 6 d treatment.