胆木茎的生物碱类成分研究

Alkaloids from stems of Nauclea officinalis

目的研究胆木Nauclea offcinalis茎枝生物碱类化学成分。方法综合采用硅胶柱色谱、反相柱色谱及制备HPLC色谱等方法进行分离纯化,利用紫外、质谱及核磁共振谱等方法鉴定化合物的结构。结果从胆木茎枝70%乙醇提取物中分离得到13个化合物,分别鉴定为3-R-3,4-二氢牛眼马钱托林碱(1)、吐叶醇(2)、naucleofficine D(3)、1,2,3,4-四氢-β-咔啉(4)、3-S-3,4-二氢牛眼马钱托林碱(5)、latifoliamide D(6)、latifoliamide B(7)、牛眼马钱托林碱(8)、3,14-二氢狭花马钱碱(9)、3,14,18,19-四氢狭花马钱碱(10)、6′-乙酰基异长春花苷内酰胺(11)、喜果苷(12)、异长春花苷内酰胺(13)。结论化合物2和4为首次从该属植物中分离得到,化合物6、7、9、10为首次从该植物中分离得到。

Objective To study the alkaloids from the stems of Nauclea officinalis. Methods The chemical constituents were separated and purified by silica gel, ODS column chromatography, and preparative HPLC. Their structures were determined by UV, MS, and NMR spectroscopic analyses. Results Thirteen compounds were isolated from the stems of N. officinalis, the structures were identified as 3-R-3,4-dihydroangustoline（1）, blumenol A（2）, naucleofficine D（3）, 1,2,3,4-tetrahydro-β-carboline（4）, 3-S-3,4-dihydroangustoline（5）, latifoliamide D（6）, latifoliamide B（7）, angustoline（8）, 3,14-dihydroangustine（9）, 3,14,18,19-tetrahydroangustine（10）, 6′-acetyl-strictosamide（11）, vincosamide（12）, and strictosamide（13）. Conclusion Compounds 2 and 4 are obtained from the plants of Nauclea L. for the first time. Compounds 2, 4, 6, 7, 9, and 10 are obtained from N. officinalis for the first time.