干酪乳杆菌L9对健康人群肠道健康的影响

Effect of Lactobacillus casei L9 on Gut Health of Healthy Individuals

目的:研究干酪乳杆菌L9在公共假期期间对健康人群肠道菌群的调节和肠道健康改善作用。方法:共募集40名健康成人,随机分成两组,其中对照组20人,试验期间正常饮食,不饮用发酵乳制品;实验组20人,1周排空期后,饮用L9样品(含L9活菌制剂的植物蛋白饮料,活菌含量约为1.0×108CFU/m L)两周,服用剂量为100m L/d。每周采集1次粪便样品,利用定量PCR分析肠道菌群的变化,并以调查问卷的形式分析人群的排便、肠道健康状况。结果:试验组粪便形状的BS(Bristol Stool Form Scale)分数与对照组相比显著增加(P<0.05),表明饮用L9利于软便的产生,缓解便秘。与假期前排空期相比,对照组假期后跟踪期肠道潜在致病菌肠球菌属和大肠杆菌的数量显著升高(P<0.05),有益菌双歧杆菌属的数量显著下降(P<0.05),说明假期的饮食对肠道菌群产生显著影响。与对照组和排空期相比,饮用L9后(试验组),大肠杆菌的数量显著下降(P<0.01),1周后梭菌属XIVa和XIVb类群数量发生极显著性降低(P<0.01),有益菌乳杆菌属的数量显著升高(P<0.01)。与排空期相比,试验组饮用期双歧杆菌属的数量显著升高(P<0.05)。本试验结果表明,L9具有调节肠道菌群,改善肠道健康的作用。

During the public holiday, intestinal disorders phenomenon such as diarrhea or constipation caused by surfeit and diet imbalance is very common in China. Probiotic could modulate intestinal microflora and improve gut health. In this study, a parallel control human trial was performed to evaluate the effect of Lactobacillus casei L9 on human intestinal microflora and intestinal health during the public holiday. A total of 40 healthy adults were assigned to two groups randomly. There were 20 people in the control group, who continued their normal diet with exclusion of dairy fermented products. Another 20 subjects were in treatment group, who successfully complete the one week baseline period entered ingestion period. During ingestion period, subjects drank one bottle（100 m L） of the study product（vegetable protein drink containing 1.0×10^8CFU/m L L9） for two weeks. Fecal samples were collected once every week. The change of intestinal microflora were analyzed by real-time PCR. The change in stool consistency was recorded in subject daily.The results showed that BS（Bristol Stool Form Scale） score of treatment group was significantly higher than the score of control group（P〈0.05）, which indicated that L9 could increase the softness of stool, and improve constipation. In following-up period, the control group had higher number of potentially pathogenic Enterococcus genus and Escherichia coli（P〈0.05） and lower number of beneficial Bifidobaceterium（P〈0.05）, compared with baseline period. That indicated intestinal microflora changed significantly during holiday. Compared to control group and baseline period, significant lower in potentially pathogenic Escherichia coli（P〈0.01） and increase in beneficial Lactobacillus（P〈0.01） were abserved in treatment group in ingestion period. Compared to following-up period, treatment group had higher number of Bifidobaceterium in ingestion period（P〈0.05）. These results indicated that Lactobacillus casei L9 could modulate intestional microflora and improve gut health of human during the public holiday.