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一株牛副流感病毒3型的分离鉴定

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摘要 牛副流感病毒3型是引起牛呼吸道疾病的主要病原之一。本研究从河南省某牛场具有急性呼吸道症状的牛鼻腔棉拭子中,应用牛肾脏细胞MDBK进行病毒分离,分离的病毒能吸附并凝聚豚鼠红细胞,血凝效价为1:32。对病毒进行提取RNA,反转录后PCR扩增HN基因,测序结果显示,分离病毒序列为牛副流感病毒3型。
作者 张忠玉
出处 《中国畜禽种业》 2016年第9期106-107,共2页 The Chinese Livestock and Poultry Breeding
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参考文献9

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