口腔鳞癌侵袭方式与α-SMA、胶原纤维表达的相关性对预后的影响

Correlation of Collagen Fiber and α-SMA Expression with Invasion Pattern and Prognosis of Oral Squamous Cell Carcinoma

[目的]检测肌成纤维细胞标志物α-SMA和胶原纤维在人口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)的表达情况,探讨它们的表达与癌侵袭方式等级积分(Invasive pattern grading score,IPGS)间的关系及对预后的影响。[方法 ]采用HE染色判定侵袭方式等级积分;Masson染色和免疫组化分析胶原面积百分比(collagen area percentage,CAP)和α-SMA的阳性百分率,并进行统计学分析。[结果]80例OSCC样本在IPGS<5组和≥5组的病例数分别为32和48例。α-SMA强阳性比例和CAP在IPGS<5组和≥5组存在差异,分别为P=0.004,P=0.001;Spearman分析验证了α-SMA与CAP呈负相关(r=-0.391)。IPGS<5患者生存时间长于IPGS≥5患者(P=0.001)。[结论]α-SMA在IPGS≥5的OSCC患者中的表达显著强于IPGS<5患者,胶原纤维表达与肌成纤维细胞的出现呈负相关。在OSCC中IPGS联合α-SMA和胶原纤维的表达可以作为OSCC患者的临床预后判断和诊断检测有价值的指标。

[Objective] To investigate the expressions of collagen and muscle fibroblasts marker α-SMA in human oral squamous cell carcinoma（OSCC）,and their relation to invasive pattern and disease prognosis. [Methods] The invasive pattern grading score（IPGS） was examined by HE staining,the collagen fiber area percentage（CAP） was determined by Masson staining and the expression of α-SMA was detected by immunohistochemistry in 80 tissue specimens of OSCC. [Results] In 80 cases of OSCC,there were 32 cases with IPGS〈5 and 48 cases with IPGS≥5. There were significant differences in strongly positive rate of α-SMA or CAP between IPGS〈5 group and ≥5 group（P=0.004,P=0.001,respectively）. Spearman analysis showed that there was a negative correlation between CAP and α-SMA（r=-0.391）. The survival in IPGS〈5 group was longer than that in IPGS≥5 group（P=0.001）. [Conclusion] The results indicate that IPGS combining with α-SMA and collagen might be used as indicators for assessing clinical diagnosis and prognosis of OSCC.