Beagle犬良性前列腺增生模型的制作及介入栓塞方法探讨

Establishment of a Beagle canine model and transarterial prostate embolization of benign prostatic hyperplasia

目的介绍运用丙酸睾酮(testosterone propionate,TP)药物诱导制作Beagle犬良性前列腺增生动物模型,并探讨介入栓塞方法的运用。方法实验对象为雄性Beagle犬16只,随机分为2组:模型组(n=8)、对照组(n=8)。其中模型组去势1个月后给予TP 2.5 mg/kg/d肌肉注射,连续3个月。16只Beagle犬均行MRI检查观察前列腺体积,分别于去势后1个及4个月行MRI扫描。4个月后模型组行股动脉插管造影观察双侧前列腺供血动脉,并行前列腺动脉栓塞,根据选用导管类型分为2组:罗伯特导管(Roberts uterine catheter,RUC)组(n=4)、眼镜蛇导管(Cobra)组(n=4)。结果模型组Beagle犬去势后运用TP药物诱导3个月后均成功建立良性前列腺增生模型,其与去势前相比体积均显著增大(P<0.05),对照组前列腺体积无显著变化(P>0.05)。模型组前列腺动脉均明确显示,血管增粗。RUC组插管成功率、超选择栓塞率高于Cobra组,手术时间短于Cobra组。结论运用TP药物诱导Beagle犬良性前列腺增生是前列腺增生介入治疗实验研究较为理想的动物模型。MRI能准确评估前列腺的体积变化。股动脉插管后用RUC导管血管造影观察双侧前列腺动脉成功率高,可完成超选择栓塞,值得推广。

Objective To establish a Beagle canine model of testosterone propionate （TP）-induced benign prostatic hyperplasia （BPH） and to assess the feasibility of transarterial prostate embolization （TAE） for reducing the prostate volume in TPinduced BPH model. Methods The study was approved by the institutional ethics committee for animal research. Sixteen beagle dogs were randomly assigned to the embolization group （n=8） or control group （n=8）. The Beagle dogs in the embolization group were castrated by an urologist. Intramuscular Benzylpenicillin （1,600,000 IU） was injected daily for 3 days to prevent infection. After 4 weeks, 2.5 mg/kg of TP were injected intramuscularly daily for 3 months. The prostate volume of all dogs was measured on MRI before and at 3 months after initiation of hormone administration. The 8 dogs in the embolization group underwent TAE 3 months after initiation of TP using Roberts uterine catheter （4） or Cobra catheter （4）. Results The canine BPH model was successfully established in all 8 Beagle dogs. The mean volume of the prostate increased significantly after 3 months of hormone administration. Digital subtraction angiography （DSA） clearly depicted dilatation of the prostate arteries after 3 months of hormone administration. The success rate and duration of super-selective catheterization and TAE using Roberts uterine catheter was significantly higher and shorter than that of Cobra catheter. Conclusion A Beagle canine model of TP-induced BPH is successfully established and is ideal for interventional experiment. The change in prostate volume can be accurately depicted on MRI. Superselective catheterization and TAE using Roberts uterine catheter is better than that of Cobra catheter.