植物乳杆菌发酵培养基的优化

Optimization of Fermentation Medium for Lactobacillus plantarum

[目的]在成功筛选出1株有强产酸能力的植物乳杆菌的基础上,优化其发酵培养基,以提高其生物量。[方法]使用单因素试验和正交试验法对培养基的成分进行优化。[结果]优化后的发酵培养基为:蔗糖30.00 g/L,酵母浸膏50.00 g/L,无水乙酸钠5.00 g/L,磷酸氢二钾2.00 g/L,柠檬酸氢二铵2.00 g/L,硫酸镁0.58 g/L,硫酸锰0.25 g/L,吐温-80 1 m L/L,碳酸钙2.00 g/L。经优化后植物乳杆菌发酵液的OD值从5.701增长到15.021,活菌数达7.1×109cfu/m L。[结论]优化后的植物乳杆菌发酵培养基降低了生产成本,为后续工业化生产的研究提供了参考。

[Objective] To optimize the fermentation medium, and to enhance the biomass of Lactobacillus plantarum based on successful screening of a L.plantarum with strong acid production.[Method] The single factor test and orthogonal test were used to optimize the composi-tion of the culture medium.[Result] The optimized fermentation medium was as follows：30.00 g/L sucrose, 50.00 g/L yeast extract, 5.00 g/L anhydrous sodium acetate, 2.00 g/L dipotassium hydrogen phosphate, 2.00 g/L diammonium hydrogen citrate, 0.58 g/L magnesium sulfate, 0.25 g/L manganese sulfate, 1 mL/L Tween-80, and 2.00 g/L calcium carbonate.OD value of L.plantarum broth after optimization increased from 5.701 to 15.021, viable count reached 7.1 ×10^9 cfu/mL.[Conclusion] The production cost was reduced by the optimized fermentation medium, which provided reference for the study of the subsequent industrial production.