空间诱变后嗜酸乳酸菌对小鼠肠道功能的影响

Effects of Lactobacillus acidophilus after Space Mutation on Gastrointestional Function in Mice

[目的]利用空间诱变技术处理嗜酸乳酸菌(Lactobacillus acidophilus),探讨空间诱变后菌株对小鼠肠道功能的影响。[方法]40只昆明小鼠适应性喂养7 d后随机分为4组:诱变前菌株组(SBM组)、诱变后菌株组(SAM组)、合生元益生菌组(BP组)和空白对照组(NC组),每组各10只。通过灌喂不同的菌悬液,探讨其对小鼠肠道菌群、小肠运动功能和排便的影响。[结果]SAM组小鼠粪便中的双歧杆菌和乳杆菌显著高于诱变前菌株组和空白对照组(P<0.05),肠球菌和肠杆菌显著低于诱变前菌株组和空白对照组(P<0.05),与合生元益生菌组无明显差异(P>0.05);诱变后菌株组小鼠的小肠运动功能和排便功能优于诱变前菌株组和空白对照组(P<0.05)。[结论]空间诱变后的嗜酸乳酸菌改善小鼠胃肠道功能的效果优于诱变前的乳酸菌,与市售合生元相当。

[Objective] To study the effect of Lactobacillus acidophilus after space mutation on gastroenteric function in mice.[Method] 40 Kunming male rats were equally divided into four groups randomly after 7 d, which were strain before mutagenesis group （ SBM group） , strain after mutagenesis group （SAM group）, Biostime probiotic group （BP group） and normal control group （NC group）.There were ten mice in each group.Through feeding different bacteria suspensions, we researched their effects on cacation, intestinal flora, and small intestine move-ment function of rats.[ Result] The counts of bifidobacterium and lactobacillus in SAM group were significant higher than SBM group and NC group （P〈0.05）, the intestine movement function and defecation function were better than SBM group and NC group （P 〈0.05）. [ Conclusion] Compared with strain before mutagenesis, Lactobacillus acidophilus after space mutation can significantly improve the gastrointes-tinal function in mice.Its efficacy is equivalent with biostime.