大八角枝叶中1个新的seco-prezizaane型倍半萜化合物

A new seco-prezizaane-type sesquiterpenoid from twigs and leaves of Illicium majus

目的研究大八角Illicius majus枝叶的化学成分。方法采用硅胶、Sephadex LH-20柱色谱、快速制备色谱和制备HPLC等技术进行分离和纯化,通过HR-ESI-MS、1D-NMR、2D-NMR等波谱数据鉴定化合物的结构。通过MTT法评价了化合物的细胞毒活性。结果从大八角枝叶提取物中分离得到1个seco-prezizaane型倍半萜,命名为大八角酮(1)。化合物1对人结肠癌细胞HCT-8、人肝癌细胞Bel-7402、人胃癌细胞BGC-823以及人肺癌细胞A549的IC50值分别为42.3、48.5、51.1和39.9μmol/L。结论化合物1为1个新的倍半萜类化合物,未表现出明显的细胞毒活性。

Objective To study the chemical constituents from the twigs and leaves oflllicius majus. Methods The compound was isolated by column chromatography over silica gel, Sephadex HL-20, and flash chromatography coupled with preparative HPLC. The structure was elucidated by spectroscopic analysis including HR-ESI-MS, 1D-NMR, and 2D-NMR. The cytotoxic activities were assessed by MTT. Results A new seco-prezizaane-type sesquiterpenoid named majusatone （1） was isolated from the twigs and leaves ofL majus. Compound 1 exhibited the cytotoxic activity against HCT-8, Bel-7402, BGC-823, and A549 with IC50 values of 42.3, 48.5, 51.1, and 39.9 μmol/L, respectively. Conclusion Compound 1 is a new special seco-prezizaane-type sesquiterpenoid, and shows no significant cytotoxic activities against HCT-8, Bel-7402, BGC-823, and A549.