贵长猕猴桃多糖提取工艺及体外抗氧化功能

Extraction and Antioxidant Activity of Polysaccharides from Guichang Kiwifruit

研究贵长猕猴桃果肉多糖、果皮多糖的提取工艺及体外抗氧化能力。采用正交试验优化贵长猕猴桃果肉、果皮多糖提取工艺参数,蒽酮-硫酸法测定多糖含量;用邻苯三酚自氧化及Fenton反应法测定贵长猕猴桃多糖抗氧化活性。结果表明:果肉多糖最优提取工艺参数为提取温度80℃、提取时间2 h、料液比1∶35(g/mL),重复提取3次,多糖提取率可达1.74%;果皮多糖最优提取工艺参数为提取温度70℃、提取时间2 h、料液比1∶25(g/mL),重复提取3次,多糖提取率可达1.16%。贵长猕猴桃果肉粗多糖、果皮粗多糖对O_2^-·和·OH均具有很好的清除作用,果肉多糖清除O_2^-·和·OH的IC_(50)分别是12.4、41.3μg/mL,果皮多糖清除2种自由基的IC_(50)分别是84.1、50.8μg/mL。

The extraction and antioxidant activity of polysaccharides from Guichang kiwifruit pulp or pericarp wereinvestigated in this work. The optimization of the extraction parameters was investigated by using orthogonal array design.Polysaccharide content was determined by the anthranone-sulfuric acid method. The antioxidant activities of Guichang kiwifruitpolysaccharides were tested by pyrogallol autoxidation and Fenton’s oxidation reaction. The results showed that the optimumextraction conditions for kiwifruit pulp polysaccharides were found to be 2 h extraction at 80 ℃ with a solid-to-solvent ratio of1：35 （g/mL） performed three times, and those for kiwifruit pericarp polysaccharides were determined as follows： three extractioncycles at 70 ℃ each for 2 h at a solid-to-solvent ratio of 1：25 （g/mL） . Under the optimized conditions, the yield of polysaccharideextracted from kiwifruit pulp and pericarp reached 1.74% and 1.16%, respectively. The polysaccharides from kiwifruit pulp andpericarp both had strong scavenging effects on superoxide anion and hydroxyl radicals with half inhibitory concentration （IC50） of12.4 and 41.3 μg/mL for pulp polysaccharides and 84.1 and 50.8 μg/mL for pericarp polysaccharides, respectively.