手十二井穴刺络放血对急性一氧化碳中毒意识障碍大鼠促醒作用及其机制的研究

Study on the mechanism of promoting awakening of twelve well points of the hand pricking blood therapy on acute carbon monoxide poisoning consciousness disorder rats

目的:观察探讨手十二井穴刺络放血对急性一氧化碳中毒大鼠意识状态、静脉血中碳氧血红蛋白(COHb)浓度及脑组织中谷胱甘肽过氧化物酶(GSH-Px)活性的影响。方法:选用成年雄性SD大鼠30只,将其随机分为正常组、模型组及治疗组3组,每组10只。于大鼠染毒前、染毒后即刻、染毒后1 h及4 h,观测大鼠意识状态,分别取大鼠尾静脉血检测COHb浓度,4 h后取脑组织,进行GSH-Px活性检测。结果:手十二井穴刺络放血可以改善急性一氧化碳中毒大鼠的意识状态,缩短昏迷时间;染毒后1 h和染毒后4 h,治疗组大鼠静脉血中COHb浓度明显低于模型组,三组比较均有差异(F=10.640,F=4.401;P<0.05)。染毒后4 h,治疗组大鼠脑组织中GSH-Px活性明显高于模型组,三组比较均有差异(F=17.626,P<0.05)。结论:手十二井穴刺络放血对急性一氧化碳中毒大鼠具有脑保护作用。

Objective To observe the effect of needling hand twelve wells phlebotomypoisoning glutathione peroxidase activity of rat consciousness, venous blood carboxyhemoglobinconcentration and brain tissue of acute carbon monoxide. Methods： A total of 30 adult maleSD rats were randomly divided into normal group, model group and treatment group, with 10rats in each group. In rats exposed before and immediately exposed after 1 hour and 4 hours, we observed the stateof consciousness of rats and tested venous blood carboxyhemoglobin contents. The brain tissue was taken out after4 hours, to detect glutathione peroxidase activity. Results： Twelve well points of the hand pricking blood in rats withacute carbon monoxide poisoning can improve the state of consciousness and shorten the duration of coma. Afterexposed for 1 hour and 4 hours, carboxyhemoglobin concentration in rat tail vein blood in the treatment group wassignificantly higher than that in the model group. The glutathione peroxidase activity in the brain tissue of the treatmentgroup was significantly higher than that of the model group after exposed for 4 hours. Conclusion： Twelve well pointsof the hand bloodletting has protective effect on brain of rats with acute carbon monoxide poisoning coma.