固有免疫功能相关的microRNAs在布鲁氏菌病患者中的表达情况

Expression pattern of innate immunity related microRNAs in human brucellosis

目的:研究与固有免疫相关的14种microRNAs在布病患者中的表达情况。方法:采用实时定量PCR方法,测定布病患者和健康志愿者血清中microRNAs的表达水平。结果:在检测的14种microRNAs中,13种在布病患者中表达下调,其中布病患者和健康志愿者之间的Ct值差异>1的microRNAs共有9种,并且miR-122、miR-145a、miR-155、miR-301a和miR-146a这5种microRNAs在布病患者血清中的表达水平明显降低(P<0.01)。结论:布病患者中miR-122、miR-145a、miR-155、miR-301a和miR-146a的表达被明显抑制,在布病诊断中具有潜在的应用价值。

Objective：To investigate the expression pattern of 14 innate immunity related microRNAs（miRNAs） in brucellosis patients.Methods：By using Real time PCR assay,expression levels of 14 miRNAs in serum samples from brucellosis patients and healthy volunteers were analyzed.Results：Of the 14 miRNAs tested,13 showed decreased expression in brucellosis patients.And significant Ct differences over one were observed for nine of the miRNAs.In them,five miRNAs,including miR-122,miR-145 a,miR-155,miR-301 a and miR-146 a levels significantly decreased in brucellosis patients（P〈0.01）.Conclusion：Decreased serum levels of miR-122,miR-145 a,miR-155,miR-301 a and miR-146 a are confirmed and may serve as novel biomarkers for human brucellosis diagnosis.