慢性乙型肝炎患者外周血NKG2A+NK细胞与Treg的关系研究

Clinical correlation between NKG2A + NK cells and regulatory T cells in patients with chronic hepatitis B virus infection

目的:探讨慢性乙型肝炎病毒(CHB)患者外周血NKG2A+NK细胞与调节性T细胞(Treg)的关系及临床意义。方法:收集46例CHB患者和17例健康对照者外周血,采用实时荧光定量PCR法检测血清HBV DNA;采用流式细胞术检测NKG2A+NK细胞及Treg的比例。结果:将CHB患者分为Low HBV DNA组(300~10~4U/ml)及High HBV DNA组(10~5~10~8U/ml)。我们发现High HBV DNA组ALT明显高于Low HBV DNA组(P<0.05)。High HBV DNA组CD56^(dim)NK细胞及NKG2A+CD56^(dim)NK细胞的比例均分别明显高于Low HBV DNA组(P<0.05)。且High HBV DNA组Treg明显高于Low HBV DNA组和对照组(P<0.05)。此外,NKG2A+CD56^(dim)NK细胞的比例与High HBV DNA载量及Treg水平均呈正相关性(r=0.59,P<0.05;r=0.53,P<0.05)。结论:CHB患者的NKG2A+CD56^(dim) NK细胞的水平与HBV的免疫逃逸及疾病的进展相关。

Objective：To investigate the correlation between NKG2 A ＋ NK cells and regulatory T cells in peripheral blood of patients with chronic hepatitis B virus infection,and explore the clinical significances.Methods：Forty-six patients with chronic hepatitis B virus infection and 17 health individuals were included in this study.HBV DNA levels were measured by Real-time quantitative PCR（FQ-PCR）.NKG2 A ＋ NK cells and Treg in PBMC were quantitatively analyzed by flow cytometry.Results：According to HBV DNA levels,the CHB patients were divided into two groups：Low HBV DNA group（Low viral load group,300-10~4 U/ml） and High HBV DNA group（High viral load group,10~5-10~8 U/ml）.We found that ALT levels of High HBV DNA group were obviously higher than Low HBV DNA group（P 0.05）.The frequenies of CD56^（dim）NK cells of High HBV DNA group were obviously higher than low HBV DNA group（P 0.05）,and similarly the percentages of NKG2 A ＋ CD56^（dim） NK cells of High HBV DNA group were significantly higher than Low HBV DNA and control groups（P 0.05）.We also found that the percentages of regulatory T cells（Treg） of High HBV DNA group were significantly higher than Low HBV DNA and control groups（P 0.05）.In addition,the proportions of NKG2A＋ CD56 ^（dim）NK cells were positively correlated with High HBV DNA levels（r = 0.59,P 0.05） and the percentages of Treg（r = 0.53,P 0.05） in CHB patients.Conclusion：NKG2 A ＋ CD56^（dim）NK cells may closely relate to the HBV-related immune escape and the progress of CHB.