免疫性血小板减少症患者外周血Treg细胞microRNA分析

Analysis of microRNA expression profile of peripheral Treg cells in ITP

目的:分析ITP患者和健康对照组Treg细胞中miRNA的表达谱的差异,探讨ITP的发病机制。方法:抽取ITP患者组和健康对照组外周静脉血,应用流式细胞仪分选Treg细胞,提取每组患者Treg细胞中的RNA,采用Solexa深度测序法对其miRNA表达谱进行分析。对比ITP组和正常对照组芯片结果,找出差异表达的miRNA,并对其进行富集性分析,找寻异常信号通路。结果:我们筛查到ITP患者组Treg细胞中存在多个miRNA表达异常,其中有显著差异者为miR-1976,miR-548ae-5p、miR-5096-p3、miR-548am-5p、PC-3p-63471、PC-3p-96627。通过富集性分析发现Erb B和TGF-β两个异常信号通路。结论:ITP患者外周血Treg细胞内miRNA表达谱存在差异表达,这些差异表达的miRNA可能影响了Treg细胞发挥正常的免疫调节功能,是ITP发生的机制之一。

Objective：To determine whether there were alteration of miRNA expression in peripheral Treg cells,through the contrast between normal people and patients with ITP and to explore the pathogenesis of ITP.Methods：Peripheral blood was obtained from ITP patients and normal people,and Treg cells were isolated by flow cytometry.RNA was extracted from Tregs in each group,then for miRNA arrays analysis through Solexa sequencing.Compare ITP group and normal control group microarray to identify differentially expressed miRNA.Differentially expressed miRNA were analyzed to find the abnormal signaling pathways.Results：We screened multiple miRNA abnormal expression in Treg cell of ITP patients,which has significant differences were miR-1976,miR-548ae-5p,miR-5096-p3,miR-548am-5p,PC-3p-63471,and PC-3p-96627.By enrichment analysis,we found two abnormal signaling pathways：Erb B signaling pathway and TGF-β signaling pathway.Conclusion：Differentially expressed miRNA were found in peripheral Treg cell of ITP patients,which may be affected the normal functioning immune regulation of Treg cells,and involved in the pathogenesis of ITP.