抗病毒治疗后HIV感染者NKT样细胞衰老和体外增殖研究

Study on senescence and proliferation of NKT like cells in HIV infected patients after antiviral therapy

目的:研究高效抗逆转录病毒治疗(Highly active antiretroviral therapy,HAART)后NKT样细胞衰老和体外增殖情况。方法:选取未接受HAART治疗、接受HAART治疗的HIV感染者以及健康人的外周血细胞,利用流式细胞仪检测接受HAARRT治疗前后的HIV感染者NKT样细胞CD57的表达情况以及体外增殖能力。结果:HIV感染者在HAART治疗前NKT样细胞的百分数显著低于健康对照组(P<0.01),HAART治疗后恢复(P<0.05);HAART治疗前NKT样细胞CD57的表达明显高于健康对照组(P<0.01),HAART治疗后恢复(P<0.05);HAART治疗前NKT样细胞体外增殖能力明显低于健康对照组,HAART治疗后有所恢复。结论:经过HAART治疗后,HIV感染者NKT样细胞的数量、CD57表达以及体外增殖能力有所恢复。

Objective：To better understand the changes of the NKT like cells after HIV infection and HAART treatment.Methods：Peripheral blood from HIV-infected individuals,HAART-treatment AIDS patients and healthy controls were collected,the expression of CD57 and the proliferation ability of NKT like cells before and after HAART were analyzed by flow cytometry.Results：We found that the percentage of NKT like cells before HAART was significantly lower than the healthy controls（P〈0.01）,and recovered after HAART treatment（P〈0.05）;the aging of NKT like cells was significantly higher before HAART compared with health individuals（P 0.01）,and recovered after HAART treatment（P〈0.05） the proliferation was significantly lower in vitro before HAART compared with healthy controls,and partial recovered after HAART.Conclusion：After HAART treatment,the number of NKT like cells,CD57 expression and the proliferation ability of HIV infected patients were restored.