珲春野生玫瑰的组织培养试验

Tissue Culture Test of Chunhun Wild Rose

为了进一步完善野玫瑰组培快繁技术体系,试验以珲春野玫瑰为试材,通过继代培养,建立野玫瑰的组培繁育体系。结果表明,适宜野玫瑰组培苗继代增殖的培养基配方为改良MS+IBA 3.0mg/L+ZT 1.5 mg/L,增殖倍数达5.21,加入椰乳后,增殖倍数和株高均显著提高,而且叶片密集,分枝明显增多,较适宜的椰乳浓度为150 ml/L;适合野玫瑰组培苗生根的培养基配方为1/4改良MS+IBA 5 mg/L+AC 1.5 g/L,生根率达88.89%,明显缩短生根周期。活性炭对野玫瑰组培苗生根率的影响不显著,但可以改善根际的通气条件,使根系数量和长度均有一定程度的提高,植株长势较好。

Aiming at the improvement of tissue culture rapid propagation on wild Rosa rogusa Thunbll, this study set up tissue culture propagation system by using subculture and wild Rosa rogusa Thunbll as the test material. The results showed that： the suitable proliferated medium for wild rose tissue culture plantlets was modified MS＋IBA 3.0 mg L-1 ＋ ZT 1.5 mg L-1, and proliferation times reached to 5.21. After adding coconut milk, the proliferation multiple and plant height were significantly increased, and the leaves were more dense, and the branches increased obviously. The best concentration of coconut milk was 150 ml L-1. The suitable medium for the rooting of tissue culture seedling of wild Rosa rogusa Thunbll was 1/4 modified MS ＋IBA 5.0 mg L-1 ＋AC 1.5 g L-t, and the rooting rate reached to 88.89% , which shorten the rooting culture periods of wild Rosa rogusa Thunbll. Activated carbon had little effect on the rooting rate of the tissue culture seedling, but it could improve the aeration status of the rhizosphere, and increase the number and length of the root in some extent which lead to better growth of the plant.