hBMSCs幼年食蟹猴脑内移植的安全评价、迁移及分布

Safty evaluation,migration and distribution of human bone marrow derived-mesenchymal stem cells in the cns of young macaca fascicularis

目的评价h BMSCs幼年食蟹猴脑内移植的安全性,并进一步观察h BMSCs在脑内的迁移和分布情况,为治疗儿童中枢神经系统疾病建立新的技术平台及理论依据。方法直接向幼年性食蟹猴脑内注射h BMSCs,通过HE染色和免疫组化的方法观察注射部位炎症反应及免疫排斥情况;通过对h BMSCs体外标记示踪和携带的男性特异基因的检测,观察移植后h BMSCs在脑内的迁移及分布情况。结果研究结果显示直接脑内注射h BMSCs未引起受试动物出现全身症状,移植部位脑组织炎症反应局限,未见明显免疫排斥反应,未见脑组织变性坏死和明显胶质细胞增生聚集。h BMSCs移植4周后,仍大量存活,并可在脑内广泛迁移,其迁移分布具有一定的规律性,可观察到不同移植受体细胞分布重叠现象。另外,h BMSCs迁移分布具有从前脑向后脑迁移及向血管和室管膜下迁移的趋势。结论 h BMSCs直接脑内注射移植安全性好;h BMSCs可以在脑内长期存活并迁移。

Objective To evalutate the safty of h BMSCs transpalntation and to observe their migration and distribution in the brain of young macaca fascicularis. To establish a new technology platform and theoretical basis for the treatment of central nervous system diseases in children. Methods Labelled h BMSCs were transplanted into the striatum of young macaca fascicularis. Brain sections were examined to evalutate the inflammatory reaction and immunological rejection of local injection sites by HE observation and immunohistochemical staining. Migration and distribution of transplantedh BMSCs was observed by real-time fluorescence quantitative PCR of male DNA and fluorescence microscope. Results The results showed that the direct intracerebral injection of h BMSCs did not cause systemic symptoms in animals. There is no inflammatory reaction and immunological rejection was detected,and degeneration and necrosis of neural cells andproliferation of glial cells were absent in the local injection sites. The transplanted h BMSCs survived,and migrated into the brain after 4 weeks transplantation. Its migration and distribution have certain regularity and were overlapping between transplant recipients. In addtion,h BMSCs tended to extend rostrally into the forebrain and showed preference of migrating toward the blood vessels and below the ependyma. Conculsions Intracerebral transplantation of h BMSCs is safe. And h BMSCs can survive and migrate into the brain.