多光谱消色差成像流式细胞仪的光学系统设计

Optical System Design of Multispectral Achromatic Imaging Flow Cytometer

为了解决成像流式细胞仪多色、宽波段、长工作距离以及大数值孔径等难点，设计了1款多光谱成像流式细胞仪光学系统。系统基于模块化设计思想，灵活运用部分和整体的优化方法，保证各模块的相对独立性；显微物镜引入衍射元件对宽谱段色差进行校正，保证在全波段下成像质量均达到理想的性能指标；多光谱分解镜组采用二向色镜堆栈分光，多重结构同时优化6个通道，大大降低了系统对色差的校正难度。全局优化得到最终的光学系统，放大倍率为60倍，视场为60μm×128μm，波段为420～800nm，6个分光通道，分辨率达到0．5μm，成像质量接近衍射极限。

In order to solve the difficulties of the multi-color, wide wavelength band, long working distance and high numerical aperture in imaging flow cytometer, an optical system of multispectral achromatic imaging flow cytometer is designed. Based on the modular design concept, the relative independence of each module is guaranteed by the flexible use of the local and whole optimization method. The microscope objective introduces diffractive optical element to correct chromatic aberration in the wide spectrum. So the imaging quality can meets ideal performance in full-wave band. The multispectral group uses dichroscope stack to split light, and six channels are optimized by multiple structure. The difficulty of chromatic aberration correction is reduced greatly. The final optical system is got by global optimization. The magnification is 60, the field of view is 60 μm× 128 μm, the wavelength range is from 420 nm to 800 nm, the system has six channels, the resolution is 0.5 μm, and the imaging quality is close to diffraction limit.