添加剂提高酶稳定性研究进展

Advance in enzymes stability enhancement through additives supplementing

酶作为一种高效的生物催化剂,以其独特的优势广泛应用于化工、医药、食品、能源等各领域。然而,大多数酶在反应时会遇到高温、高盐、极端p H等逆境,容易造成酶的失活,进而降低生产效率,这严重限制其在工业上的推广和应用。因此,强化酶稳定性已成为当前研究的热点和难点。添加稳定剂是一种简单、经济、有效的提高酶稳定性的方法,对几种不同类型添加剂的特点及其在改善酶稳定性方面的应用作了综述,并从结构和功能的角度进一步解释了相关机理。

As highly efficient biocatalysts, enzymes have been widely used in the fields of chemical, pharmaceutical, food and energy industry with outstanding advantages. However, most enzymes face adverse conditions during the bioreactiou, including high temperature, high salinity and extreme pH, and are very labile to lose activity, which severely limits their industrial applications. Therefore, it is critical and also challenging to enhance enzyme stability. Stabilizer additives supplementing is efficient to improve enzymes＇ stability. The characteristics of several different types of additives and their application in enzymes＇ stability enhancement were reviewed, and the underlying mechanisms were elucidated at the level of structure-function relationship.