浸润性乳癌组织中miRNA-34a甲基化状态分析

METHYLATION STATUS OF MICRO-RNA-34a IN INVASIVE BREAST CANCER

目的了解浸润性乳癌组织中miRNA-34a启动子区CpG岛甲基化状态,并分析其临床意义。方法采用甲基化特异性PCR（MSP）技术,检测56例浸润性乳癌病人癌组织及相应癌旁正常乳房组织（距病灶边缘〉5cm）中miRNA-34a上游启动子区CpG岛甲基化情况,并分析其与各临床病理参数之间的关系。结果浸润性乳癌组织中miRNA-34a启动子区甲基化率显著高于癌旁正常乳房组织,差异有显著性（χ~2=5.630,P〈0.05）。浸润性乳癌组织中miRNA-34a启动子区甲基化水平与乳癌组织学分级及腋窝淋巴结转移有相关性,差异有显著性（χ~2=10.920、8.737,P〈0.05）,而与其他临床病理参数无关。结论浸润性乳癌组织中miRNA-34a启动子区呈现髙甲基化水平,可能促进了浸润性乳癌的发生发展。对其进行检测有助于乳癌的早期诊断及预后判断。

Objective To understand the methylation status of the CpG islands around the promoter regions of miRNA- 34a in invasive breast cancer; and evaluate its clinical significance. Methods Employing methylation-spcific PCR, the methyla- tion status of miRNA-34a promoter in cancer and adjacent normal tissue （from the edge of cancer 〉 5 cm） in 56 patients with inva- sive breast cancer （IBC） were detected. The correlation between these genetic features and clinical pathology was analyzed. Re- sults The methylation rate of CpG islands around the promoter regions of miRNA-34a in IBC was higher than that in adjacent normal breast tissue （X^2=5.630,P〈0.05）. The methylation of the CpG islands around the promoter regions of miRNA 34a in IBC was correlated with its histological grade and axillary lymph node metastasis （X^2 = 10.920,8. 737;P %0.05）, but unrelated with other clinical pathology parameters. Conclusion The aberrant hypermethylation of CpG island in the promoter of miRNA-34a may promote the development and progress of invasive breast cancer, a detection of it is conducive to an early diagnosis of the cancer and prediction of its prognosis.