外周血TLR2及TLR4表达与血管性痴呆的相关性

ASSOCIATION OF PERIPHERAL BLOOD TLR2 AND TLR4 EXPRESSIONS WITH VASCULAR DEMENTIA

目的探讨中国汉族人群Toll样受体2（TLR2）和Toll样受体4（TLR4）外周血表达水平及其与血管性痴呆（VaD）的相互关系。方法采用病例-对照研究的方法,选择性别、年龄等相关因素相匹配的中国汉族VaD病人和健康人（对照组）各200例,应用聚合酶链反应（PCR）技术和流式细胞仪定量检测外周血单核细胞表面TLR2和TLR4蛋白和mRNA的表达水平。结果 VaD组TLR2和TLR4的mRNA与蛋白表达水平均明显高于对照组（t=12.30~63.50,P〈0.05）。多元线性回归分析显示,两组TLR2蛋白表达水平及TLR4的mRNA和蛋白表达水平与VaD之间存在显著相关（P〈0.05）。结论外周血中TLR2和TLR4的表达水平与VaD的发病之间存在相关关系。

Objective To investigate the association of Toll-like receptors-TLR2 and TLR4-in peripheral blood with vascular dementia（VaD）in Chinese Han population. Methods Applying case-control study,VaD patients and health Chinese Han people of gender,age and other related factors matched each other were recruited each in 200 and divided into two groups.The expressions of TLR2 and TLR4on the surface of monocytes in peripheral blood were detected using polymerase chain reaction（PCR）and flow cytometry（FCM）. Results In VaD patients,the levels of TLR2 and TLR4mRNAs were higher than that in the control group（t=12.30-63.50,P〈0.05）.Multiple linear regression showed the levels of TLR2 mRNAs and TLR4 mRNAs and proteins were closely associated with VaD（P〈0.05）. Conclusion There is a correlation of expression levels of TLR2 and TLR4 on monocytes in peripheral blood with the onset of vascular dementia.