红花黄色素对神经根型腰椎病大鼠抗炎作用及其机制研究

The anti-inflammatory effect and mechanism of saffower yellower on lumbar spondylopathy of nerve root type in rats

目的:研究红花黄色素对神经根型腰椎病大鼠抗炎作用及其机制。方法:建立神经根型腰椎病大鼠模型,随机分为假手术组、模型组、PDTC(吡咯烷二硫代甲酸铵盐)组、红花黄色素100 mg/kg、50 mg/kg、25 mg/kg剂量组。红花黄色素各组于造模后3 d静脉注射给药,共给药7 d。检测后肢热痛刺激缩爪阈值的变化;椎板法检测血液流变学指标;Elisa法检测大鼠血清中TNF-α、IL-1β、IL-6、COX-2水平;Western法检测脊髓背角中p-NF-k B P65蛋白的表达;RT-PCR法检测NF-k B P65 mRNA的表达。结果:与模型组相比,100 mg/kg、50 mg/kg、25 mg/kg剂量的红花黄色素均能显著提高热痛刺激缩爪阈值、改善血液流变学各指标;显著降低TNF-α、IL-1β、IL-6、COX-2水平;显著抑制p-NF-k B P65蛋白及NF-k B P65 mRNA的表达。结论:红花黄色素可能对神经根型腰椎病的治疗有一定的意义,其机制与抑制NF-k B P65通路的激活有关。

Objective： To observe the anti-inflammatory effect and mechanism of saffower yellower on lumbar spondylopathy of nerve root type in rats. Methods： The lumbar spondylopathy of nerve root type in rats was builded. Rats were randomly divided into sham, model, inhibitor （PDTC） and the 100 mg/kg, 50 mg/kg, 25 mg/kg dose of SY group. 3 days after model building, SY was administrated by intravenous in- jection, 14 days continuously. Thermal withdrawal latency was detected by therml allodynia. Hemorheology indexes were detedcted by rotary cone-plate viscometer. TNF-α, IL-1 β, IL-6 and COX-2 level in serum was detected by Elisa method, p-NF-kB P65 protein level in spinal dorsal horn was detected by western blot. NF-kB P65 mRNA level in was detected by RT-PCR. Results： Compared with model group, hemotheology indexes were improved significantly, Thermal withdrawal latency, TNF-α, IL-1β, IL-6, COX-2 level, p-NF-kB P65 protein and NF-kB P65 mRNA level was significantly decreased in the 100 mg/kg, 50 mg/kg and 25 mg/kg dose of SY（p 〈0.01 ,p 〈0.05）. Conclu- sion： Saffower yellower plays an important role on lumbar spandylopathy of nerve root type and the mechanism may be related with inhibiting activation of NF-kB P65 pathway.