microRNA-212对胃癌细胞系SGC7901迁移和侵袭能力的影响

Effects of microRNA-212 on migration and invasion of gastric cancer cell lines SGC7901

目的:研究人microRNA-212(miR-212)对胃癌细胞系SGC7901迁移和侵袭能力的影响。方法:通过化学方法合成人成熟型miR-212抑制基因i-miR-212,分别以25、50、75、100 nmol/L浓度通过脂质体转染方法转染SGC7901细胞,同时设空白组和无序列对照组。采用Real-time PCR、细胞计数和Transwell小室等实验方法,分别检测各组细胞miR-212的表达、miR-212对细胞增殖的影响及miR-212对细胞迁移和侵袭能力的影响。结果:各浓度i-miR-212转染SGC7901细胞后,miR-212表达均降低,以50 nmol/L i-miR-212转染组最佳,较无序列对照组降低约94.60%;50 nmol/L i-miR-212转染组与无序列对照组在细胞计数上差异有统计学意义(P<0.01)。在细胞侵袭实验中50 nmol/L i-miR-212转染组透膜细胞明显多于无序列对照组(P<0.01),在迁移实验中i-miR-212转染组(50 nmol/L)的透膜细胞明显多于无序列对照组(P<0.01)。结论:转染成熟型人i-miR-212能使胃癌细胞系SGC7901细胞中miR-212的表达明显下降,并能显著促进胃癌细胞系SGC7901的增殖、迁移及侵袭能力,表明miR-212与胃癌肿瘤细胞的转移具有相关性。

Objective： To explore the influence of different operators to the repeatability and reproducibility of Lenstar in axial length measurements. Methods： Sixty left eyes of 60 participants were enrolled. Each participant was performed two measurements which was finished by operator I and operator II, each measurement including at least three continue measure. The SD （standar deviation） and CV （coefficient of variation） of all the participants was calculated to evaluate the reproducibility of operator I and operator II. The difference between operator I and operator II in AL measurement and it’s 95%CI （confidence interval） was calculated to evaluate the reproducibility between operator I and operator II. Results： The Mean±SD of operator I and operator II were （24.05±0.02） mm and （24.05±0.02） mm. The CV of operator I and operator II were 0.07% and 0.06%. The Mean±SD of the difference of AL between operator I and operator II was （0.00±0.03） mm. The 95%CI of the difference of AL between operator I and operator II was 0.00±0.06. Conclusion： The factor of operators don’t affect the repeatability and reproducibility of Lenstar.