黄芪百合颗粒对高原低氧模型小鼠脑氧化应激损伤的影响

Protective Effect of Astragalus Lily Granules on Brain Tissue of Mice Under High Altitude Hypoxia Condition

目的探讨黄芪百合颗粒对高原低氧模型小鼠脑组织氧化应激损伤的影响。方法将60只SPF级昆明小鼠随机分为对照组、模型组、黄芪百合颗粒低、中、高(1.75、3.5、7 mg·kg^(-1))剂量组。常规饲养3 d后,灌胃给药,每日1次,连续17 d。从第14日起每日灌胃30 min后除对照组外其余各组小鼠于低氧舱中模拟高海拔低压低氧环境进行暴露,持续暴露3 d,眼球采血后处死小鼠。采用比色分析法测定脑组织中超氧化物歧化酶活性、丙二醛含量及总抗氧化能力,HE染色在显微镜下观察脑组织的形态变化进行病理评分并计数炎细胞数量。结果与对照组比较,模型组小鼠脑质量明显增加,超氧化物歧化酶活性和总抗氧化能力明显降低,脑丙二醛含量、脑组织病理评分和炎细胞数量均明显增加(P<0.01)。与模型组比较,黄芪百合颗粒中、高剂量组小鼠的脑组织超氧化物歧化酶活性和总抗氧化能力均升高,丙二醛含量、病理评分及炎细胞数均降低(P<0.05或P<0.01)。结论黄芪百合颗粒对高原低压低氧环境下小鼠脑损伤具有良好保护作用,可能是通过增强脑组织自由基清除能力实现的。

Objective To investigate the antioxidant effect of Astragalus lily granules on brain tissues of mice under high altitude hypoxia and explore the possible mechanisms. Methods Sixty KM mice were randomly divided into blank control group,model group,Astragalus lily granules low-,medium- and high- dose（ 1. 75,3. 5,7mg·kg-1） groups. After 3 d of routine feeding,the mice were administered by gavage for 17 d. From the 14 th day,all these groups except the blank control group were exposed to the high altitude hypoxia cabin for 3 successive days. Eye blood was collected before the mice were executed the next day. The superoxide dismutase（ SOD） activity,malondialdehyde（ MDA） content and total antioxidant capacity（ T-AOC） of brain tissues was determined by colorimetric assay. The morphological changes of brain tissues and the number of inflammatory cells were observed by HE staining. Results Compared with control group,cerebral index and MDA content of brain tissues in the model group were both significantly increased（ P 〈 0. 01）,so were pathological score and number of inflammatory cells in brain tissues（ P 〈 0. 01）. But T-AOC and SOD activities were both obviously decreased（ P 〈 0. 01）. Compared with the model group,SOD activities and T-AOC in brain tissues of Astragalus lily granules medium and high dose group were increased（ P 〈 0. 05 or P 〈 0. 01）,while MDA content,pathological score and number of inflammatory cells in brain tissues of Astragalus lily granules medium low and high dose group were obviously decreased（ P 〈 0. 05or P 〈 0. 01）. Conclusion Astragalus lily granules can protect the brain of hypoxic mice from injury,possibly via the metabolism of free radicals.