小麦胁迫相关基因TaSF3B2的克隆及表达特性分析

Molecular Cloning and Expression Analysis of TaSF3B2 from Wheat

为研究小麦剪接因子在逆境胁迫中的作用,通过分析小麦抗性相关EST数据库,筛选并克隆获得1条2 327bp的核苷酸序列。该序列包含了一个1 854bp的开放阅读框,编码一个由617个氨基酸残基组成的蛋白,氨基酸同源比对发现,该蛋白含有GUS1结构域(Splicing Factor 3B,Subunit 2),属于剪接因子3b亚基中的一员,将该蛋白命名为TaSF3B2。生物信息学分析显示,TaSF3B2平均亲水系数(GRAVY)为-0.895,不稳定系数为43.92,且在细胞核中发挥作用的可能性最大。实时荧光定量PCR分析表明,TaSF3B2基因在不同组织中存在差异表达,不同发育时期其表达也存在差异;在幼苗叶片中,该基因表达受高盐、低温、条锈菌、干旱及ABA激素胁迫明显下调表达,而在幼苗根中变化不明显。推测TaSF3B2基因参与了小麦正常条件下的生长发育,同时在小麦应对逆境胁迫的过程中具有重要的作用。

To study the function of splicing factor responses to abiotic stress,a cDNA sequence of TaSF3B2 was isolated and analyzed from wheat EST database.The results showed that TaSF3B2 contained a 1 854 bp ORF encoding 617 amino acids.Using the methods of bioinformatics to predict and analyze TaSF3B2,the protein has classic domain of splicing factor 3Bsubunit 2,high hydrophilia（GRAVY,-0.895）and heat stability（Instability index,43.92）.The results of real-time PCR revealed that the expression of TaSF3B2 had different levels in different developments and tissues.In addition,the expression was induced markedly by drought,salt,low-temperature,ABA and yellow rust（CY32）,with down-regulation in leaves of seedling,but no change in root.In summary,it is speculated that TaSF3B2 could play a role not only in growth and development conditions but also in biotic and abiotic stresses of wheat.