SSB1在腮腺放射性损伤修复过程中的表达变化

The expression of SSB1 in radioactive damage repair of parotid gland

目的:研究正常大鼠腮腺及放射后大鼠腮腺组织单链DNA结合蛋白(single-strand DNA-binding protein1,SSB1)的表达及变化规律,探讨SSB1与涎腺放射损伤修复的关系。方法:取近交系成年Wistar大鼠40只,一次给予6 Gy剂量照射头颈部,于放射后每小时随机处死5只动物取腮腺组织,共形成8个实验组,另取5只大鼠作为对照组。应用免疫组织化学与RT-qPCR的方法检测SSB1在大鼠腮腺组织中表达情况。结果:对照组大鼠腮腺组织有一定量的内源性SSB1表达,不同腮腺组织之间SSB1含量不同,导管>腺泡>结缔组织;放射后,大鼠腮腺组织中SSB1的表达量随着时间变化呈先增加(P<0.05)后减弱趋势,并在后期表达量降至接近正常水平。结论:辐射能诱导大鼠腮腺SSB1表达量增加,可能参与了大鼠腮腺放射性损伤修复过程。

Objective： To investigate the expression of single-strand DNA-binding protein 1（ SSB1） in rats＇ normal and irradiated parotid gland tissue,and explore the relationship between SSB1 and radioactive damage repair. Methods： Take the normal rats as control（ n = 5）,the experimental rats（ n = 40） were radiated with 60 Co gamma rays on head and neck region in a dose of 6 Gy. Five rats were randomly killed per hour to form eight experimental groups after irradiation. Immunohistochemical and RT-qPCR were applicated to detect the expression of SSB1 in rats parotid gland. Results： A certain amount of endogenous SSB1 was expressed in normal rats parotid gland tissue. The content of SSB1 was different in different parotid gland tissues,ducts acinus connective tissue. SSB1 was displayed a increasing first（ P 0. 05） and after weakening trend over time after irradiation and the unper expression was close to nomal. Conclusion： SSB1 is a radiation-induced protein,which may be involved in radioactive damage repair process in the rats＇ parotid gland.