复方苦参汤对溃疡性结肠炎模型大鼠的干预及作用机制研究

Effects of Compound Kushen Tang on Ulcerative Colitis in Rats and the Underlying Mechanism

目的:探讨复方苦参汤对溃疡性结肠炎(UC)大鼠的作用及机制。方法:采用2,4,6-三硝基苯磺酸(TNBS)诱导溃疡性结肠炎大鼠模型,用不同剂量的复方苦参汤进行干预,检测结肠组织丙二醛(MDA)、一氧化氮合酶(i NOS)和一氧化氮(NO)水平,以及髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性,观察UC大鼠一般情况和结肠组织形态变化。结果:UC大鼠结肠组织MDA(P<0.05)、i NOS(P<0.01)和NO(P<0.01)水平及MPO(P<0.01)活性显著高于正常对照组,SOD(P<0.01)和GSH-Px(P<0.05)活性显著低于正常对照组;复方苦参汤高剂量组治疗后可明显降低UC大鼠结肠组织中MPO(P<0.01)、MDA(P<0.05)、i NOS(P<0.05)和NO(P<0.01)水平,增加SOD(P<0.01)和GSH-Px(P<0.05)活性,其疗效呈剂量依赖性。同时大鼠的一般情况及结肠组织形态亦得到明显改善。结论:复方苦参汤能显著抑制UC大鼠氧化应激反应,减轻结肠炎症损伤,具有较高的临床治疗价值。

Objective： To investigate the therapeutic effects of compound Kushen Tang and its relevant mechanism in TNBS-in- duced ulcerative colitis （UC） rats. Methods： UC was induced by TNBS in rats. After compound Kushen Tang was given orally, the levels of MDA, iNOS, and NO and the activity of MPO, SOD, and GSH-Px were measured. The general condition of rats and colon tissue morphology were observed. Results： The levels of MDA （ P 〈 0.05 ）, iNOS （ P 〈 0.01 ） and NO （ P 〈 0.01 ） and the activity of MPO （P 〈0.01 ） in tissues of UC rats were significantly higher than the control group. The activity of SOD （P 〈0.01 ） and GSH- Px （ P 〈 0.05 ） were significantly lower than those in the control group. After the treatment with high doses of compound Kushen Tang, the levels of MPO （P 〈0.01）, MDA （P 〈0.05）, iNOS （P 〈0.05） and NO （P 〈0.01） were significantly decreased, and the activity of SOD （P 〈 0.01 ） and GSH- Px （P 〈 0.05） significantly increased. The therapeutic effect was dose-dependent and the general Con- dition of rats and colon tissue morphology were also significantly improved. Conclusion： Compound Kushen Tang is considered as a no- vel therapeutic alternatives for the treatment of UC, which can reduce coloni inflammatory injury and ameliorate the colitis.