地锦草-马齿苋药对的体外抑菌作用研究

Study on in vitro Antimicrobial Activity of Traditional Chinese Medicine Herbal Pair of Euphorbia humifusa Willd. and Portulaca oleracea L.

目的:研究地锦草-马齿苋药对的体外抑菌效果。方法:采用倍比稀释法和96孔板微量肉汤稀释法,考察地锦草和马齿苋药对及单味药对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌的体外抑菌作用,测定3种实验菌种的抑菌圈直径、最小抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:实验菌株对单味药材及药对呈现不同程度的敏感,其中以药对的抑杀作用最明显(P<0.05)。随温度的升高,各水提浓缩液对不同实验菌珠的抑菌活性发生改变。MIC和MBC结果显示,各水提浓缩液对大肠埃希菌的作用最强,其次为金黄色葡萄球菌,对铜绿假单胞菌抑杀作用相对较弱。结论:地锦草-马齿苋药对对实验菌株有不同程度抑杀作用,但存在一定差异,中药应用形式的变化对药对的抑杀能力有较大影响。

Objective ： To study the in vitro antimicrobial activity of traditional Chinese medicine herbal pair of euphorbia humifusa Willd. and portulaca oleracea L.. Methods： The antibacterial effect in vitro of the traditional Chinese medicine herbal pair and the single herb of euphorbia humifusa Willd. and portulaca oleracea L. was studied on staphylococcus aureus, escherichia coli and pseudo- monas aeruginosa by the 2-fold dilution method and the broth micro-dilution method in a 96-well plate. The minimum inhibitory con- centration （ MIC）, minimum bactericidal concentration （MBC） and the diameter of inhibition zone were determined. Results ： The ex- perimental strains showed the different sensitivity among the traditional Chinese medicine herbal pair and the single herb. The antibac- terial and bactericidal activity of the traditional Chinese medicine herbal pair was the most obvious （P 〈 0.05）. As the temperature in- creasing, the antibacterial activity of all water extract on different experimental strains changed. The results of MIC and MBC showed that the effects of water extract on escherichia coli were strongest, that of stapphylococcus aureus were secong, ant that of pseudomonas aeruginosa were relatively weak. Conclusion： The traditional Chinese medicine herbal pair of euphorbia humifusa Willd. and portulaca oleracea L. has antibacterial and bactericidal activity in varying degrees on the experimental strains with some differences, and the changes in the application forms of traditional Chinese medicines has great influence on the antibacterial and bactericidal ability.