HPLC法测定伤科跌打膏中姜黄素和盐酸小檗碱的含量

Determination of Berberine Hydrochloride and Curcumine in Shangke Dieda Paste by HPLC

目的:建立测定伤科跌打膏中姜黄素和盐酸小檗碱含量的方法。方法:采用高效液相色谱法。姜黄素:色谱柱:Inert Sutain C18(250 mm×4.6 mm,5μm);流动相:乙腈-4%醋酸溶液(44∶56);流速:1.0 ml·min^(-1);柱温:25℃;检测波长:430nm;进样量:10μl。盐酸小檗碱:色谱柱:Inert Sutain C_(18)(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸溶液(44∶56)(每100 ml加十二烷基磺酸钠0.1 g);流速:1.0 ml·min^(-1);柱温:25℃;检测波长:345 nm;进样量:10μl。结果:姜黄素和盐酸小檗碱分别在0.01~0.50μg(r=0.999 3)和0.02~0.26μg(r=0.999 9)范围内各自呈现良好的线性关系,平均加样回收率分别为为101.03%和99.20%,RSD分别为1.75%和0.64%(n=9)。结论:本研究建立的方法操作简单,准确灵敏,专属性强,可用于伤科跌打膏的质量控制。

Objective： To establish a method for the determination of curcumine and berberine hydrochloride in Shangke Dieda paste. Methods： An HPLC method was adopted to determine the content of curcumine and berberine hydrochloride in Shangke Dieda paste. For curcumine, the column was InertSutain C18（250 mm ×4.6 mm,5 μm） ; the mobile phase was acetonitrile and 4% acetic acid solution （44：56） ; the flow rate was 1.0 ml .min-1; the column temperature was 25℃ ; the detection wavelength was 430 nm; the sample size was 10 μl. For berberine hydrochloride, the column was InertSutain C18 （250 mm ×4.6 mm,5 μm） ; the mobile phase consisted of acetonitrile-0.1% phosphoric acid （44：56, 0.2 g dodecyl sodium sulfate was added to 100 ml solution） ; the flow rate was 1.0 ml .min-1; the column temperature was 25℃ ; the detection wavelength was 345 nm; the sample size was 10 μl. Results： A good linear correlation was obtained within the range of 0.01-0.50 μg （ r = 0. 999 3 ） for curcumin and 0.02-0.16 μg （ r = 0.999 9 ） for berberine hydrochloride. The average recovery was 101.03% with RSD of 1.75% for curcumin and 99.20% with RSD of 0.64% for berberine hydrochloride （ n = 9）. Conclusion： The established method is simple, accurate, sensitive and specific, which can be used for the quality control of Shangke Dieda paste.